GBA Antibody BIOTIN

Product no.: GLCM-BIOTIN
$375.00


General Information

 

 

Product

 

GBA Antibody BIOTIN

Description

BIOTIN-Conjugated Glucosylceramidase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Recombinant Glucosylceramidase protein.


Physical Properties

 

 

Quantity

 

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

BIOTIN-Conjugated

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20C for long term storage


Application Protocol

 

ELISA

 

1:50,000

Immunohistochemistry 1:50-1:200

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000


Protein

 

Uniprot #

Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:9201993, PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (PubMed:19279011). Also plays a role in cholesterol metabolism (PubMed:24211208, PubMed:26724485). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208, PubMed:26724485). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (PubMed:24211208). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (PubMed:24211208, PubMed:26724485).

Overview

glucosylceramidase activity

glucosyltransferase activity

hydrolase activity

scavenger receptor binding

signaling receptor binding

steryl-beta-glucosidase activity

Molecular Function

Lysosome

Subcellular Location

Interacts with saposin-C (PubMed:10781797). Interacts with SCARB2 (PubMed:18022370). Interacts with TCP1 (PubMed:21098288). May interact with SNCA; may inhibit the glucosylceramidase activity (PubMed:23266198). Interacts with GRN; this interaction prevents aggregation of GBA-SCARB2 complex via interaction with HSPA1A upon stress (PubMed:27789271).

Expression

Belongs to the glycosyl hydrolase 30 family.

Structure

Lysosomal acid glucosylceramidase

Alternative Nomenclature

Lysosomal acid glucosylceramidase

   

Protein information supplied by UniProt.

 

Accessories

Product Note Status Price
GBA Antibody GBA Antibody
GLCM-101AP $285.00 *
GBA Positive Control GBA Positive Control
PC-GLCM $200.00 *
GBA Blocking Peptide GBA Blocking Peptide
P-GLCM $155.00 *
GBA Antibody FITC GBA Antibody FITC
GLCM-FITC $375.00 *
Display accessory details