GPCR GPR43 / FFAR2 Antibody

General Information

Product

GPCR GPR43 Antibody

Description

Affinity Purified Free fatty acid receptor 2 Antibody Mid Region

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human free fatty acid receptor 2 protein.

Accession #

NCBI:  NP_005297.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O15552

Overview

G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family.

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Detected in whole wall and separated mucosa in the distal ileum and colon. Expressed by enteroendocrine cells expressing peptide YY (PYY) (at protein level).

Structure

Interacts with FCN1 (via Fibrinogen C-terminal domain).

Alternative Nomenclature

FFA2R antibody

free fatty acid activated receptor 2 antibody

Free fatty acid receptor 2 antibody

G-protein coupled receptor 43 antibody

GPR 43 antibody