General Information
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Product
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GPCR GPR43 Antibody
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Description
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Affinity Purified Free fatty acid receptor 2 Antibody Mid Region
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Verified Applications
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DB, ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 250-300 on human free fatty acid receptor 2 protein.
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Accession #
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NCBI: NP_005297.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.58-0.59 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Determinant
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Mid Region
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Immunohistochemistry |
1:50-1:100 |
Immunoprecipitation
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1:150
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Western Blot
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1:500
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Protein
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Uniprot #
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O15552
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Overview
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G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family.
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Molecular Function
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G-protein coupled receptor, Receptor, Transducer
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Subcellular Location
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Cell membrane; Multi-pass membrane protein
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Expression
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Detected in whole wall and separated mucosa in the distal ileum and colon. Expressed by enteroendocrine cells expressing peptide YY (PYY) (at protein level).
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Structure
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Interacts with FCN1 (via Fibrinogen C-terminal domain).
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Alternative Nomenclature
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FFA2R antibody
free fatty acid activated receptor 2 antibody
Free fatty acid receptor 2 antibody
G-protein coupled receptor 43 antibody
GPR 43 antibody
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