PDE10A Antibody BIOTIN

Product no.: PD10A-BIOTIN

$375.00

Description
Publications

General Information
Product	PDE10A Antibody BIOTIN-Conjugated
Description	BIOTIN-Conjugated Phosphodiesterase 10A
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide (Human) taken against a common sequence near the 250-325 amino acids downstream of N terminal region of PDE10A. Synthetic MAP.
Specificity	This antibody has no cross-reactivity against any other PDE family members tested so far. This antibody will detect all PDE10A variants and subtypes.
Accession #	GenBank: AAD32596.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.7 µg/µl in antibody stabilization buffer
Form	BIOTIN-Conjugated
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:100-1:150
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:100-1:150
Immunofluorescence	1:100-1:150
Immunohistochemistry	1:100-1:150
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9Y233
Overview	Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.
Molecular Function	Hydrolase, metal-ion binding, cGMP binding
Subcellular Location	Cytoplasm
Expression	Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
Structure	Homodimer
Alternative Nomenclature	5''-cyclic phosphodiesterase 10A antibody cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody cAMP and cAMP-inhibited cGMP 3'' antibody FLJ11894 antibody HSPDE10A antibody Phosphodiesterase 10A antibody

Protein information supplied by UniProt

Fabgennix PDE10A Antibody has been used in 14 Publications
D'Angelo, Vincenza, et al. "Phosphodiesterase-10A inverse changes in striatopallidal and striatoentopeduncular pathways of a transgenic mouse model of DYT1 dystonia." Journal of Neuroscience 37.8 (2017) PMID28115486	Immunocytochemistry, Western Blot; Mouse
Harada, Akina, et al. "Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A." Nuclear medicine and biology 42.2 (2015): 146-154. PMID25451212	Western Blot; Monkey
Mango, Dalila, et al. "Phosphodiesterase 10A controls D1-mediated facilitation of GABA release from striato-nigral projections under normal and dopamine-depleted conditions." Neuropharmacology 76 (2014): 127-136. PMID23973317	Western Blot; Mouse
Sancesario, Giuseppe, et al. "Levodopa-induced dyskinesias are associated with transient down-regulation of cAMP and cGMP in the caudate-putamen of hemiparkinsonian rats: Reduced synthesis or increased catabolism?." Neurochemistry international 79 (2014): 44-56. PMID25452081	Western Blot; Rat
Nishi, Akinori et al. “Distinct Roles of PDE4 and PDE10A in the Regulation of cAMP/PKA Signaling in the Striatum.” The Journal of neuroscience?: the official journal of the Society for Neuroscience 28.42 (2008): 10460. PMC2814340	Immunohistochemistry; Mouse
Heine, C., et al. "Phosphodiesterase 2 inhibitors promote axonal outgrowth in organotypic slice co-cultures." Neurosignals 21.3/4 (2013): 197-212. PMID22947663	Immunohistochemistry, Immunofluorescence; Rat
Giorgi, M., et al. "PDE10A and PDE10A-dependent cAMP catabolism are dysregulated oppositely in striatum and nucleus accumbens after lesion of midbrain dopamine neurons in Rat: a key step in parkinsonism physiopathology." Neurobiology of disease 43.1 (2011): 293-303.PMID21515371	Immunohistochemistry; Mouse
Ong, WK et al. “The Role of the PDE4D cAMP Phosphodiesterase in the Regulation of Glucagon-Like Peptide-1 Release.” British Journal of Pharmacology 157.4 (2009): 633–644. PMC2707975	Immunofluorescence, Western Blot; Mouse
Kruse, Lars S., et al. "PDE9A, PDE10A, and PDE11A expression in Rat trigeminovascular pain signalling system." Brain research 1281 (2009): 25-34. PMID19445908	Immunohistochemistry, Immunodepletion, Western Blot; Rat
Dlaboga, Daniel, Hassan Hajjhussein, and James M. O'Donnell. "Chronic haloperidol and clozapine produce different patterns of effects on phosphodiesterase-1B,-4B, and-10A expression in Rat striatum." Neuropharmacology 54.4 (2008): 745-754. PMID18222493	Western Blot; Rat
Bora, Roop Singh, et al. "Development of a cell‐based assay for screening of phosphodiesterase 10A (PDE10A) inhibitors using a stable recombinant HEK‐293 cell line expressing high levels of PDE10A." Biotechnology and applied biochemistry 49.2 (2008): 129-134. PMID17640173	Immunocytochemistry, Western Blot; Human
Albrecht, Knut, et al. "Immunohistochemical distribution of cyclic nucleotide phosphodiesterase (PDE) isoenzymes in the Human vagina: A potential forensic value?." Journal of forensic and legal medicine 14.5 (2007): 270-274. PMID17113813	Immunohistochemistry; Human
Ückert, Stefan, et al. "Immunohistochemical Description of Cyclic Nucleotide Phosphodiesterase (PDE) Isoenzymes in the Human Labia Minora." The Journal of Sexual Medicine 4.3 (2007): 602-608.PMID17498099	Immunofluorescence; Human
Ückert, Stefan, et al. "Cyclic adenosine monophosphate and cyclic guanosine monophosphate-phosphodiesterase isoenzymes in Human vagina: relation to nitric oxide synthase isoforms and vasoactive intestinal polypeptide-containing nerves." Urology 65.3 (2005): 604-610. PMID15780401	Immunohistochemistry; Human