PDE4D Antibody BIOTIN

Product no.: PD4D-BIOTIN

$375.00

Description
Publications

General Information
Product	Pan PDE4D Antibody BIOTIN-Conjugated
Description	BIOTIN-Conjugated Phosphodiesterase 4D Antibody
Verified Applications	ELISA, ICC/IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	C-terminal cyclic peptide unique to all PDED subtypes variants.
Specificity	This antibody labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, and PDE4D5 variants. Does not cross react with PDE4A, PDE4B, PDE4D6, PDE4D7, PDE4D8, PDE4D9, OR PDE4C.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.57 µg/µl in antibody stabilization buffer
Form	BIOTIN-Conjugated
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:150
Western Blot	1:500
Protein
Overview	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Subcellular Location	Apical cell membrane, Cytoplasm, Membrane
Expression	Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Structure	Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).
Alternative Nomenclature	cAMP specific 3',5' cyclic phosphodiesterase 4D antibody DKFZp686M11213 antibody 5''-cyclic phosphodiesterase 4D antibody cAMP specific phosphodiesterase PDE4D antibody cAMP specific phosphodiesterase PDE4D6 antibody DPDE 3 antibody Dunce like phosphodiesterase E3 antibody FLJ97311 antibody HSPDE4D antibody PDE 43 antibody PDE4DN2 antibody Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody Drosophila antibody STRK1 antibody

Protein information supplied by UniProt

	Fabgennix PDE4D Antibody has been used in 37 Publications
Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015). PMID25578602		Immunohistochemistry; *Human*
Guo, Jiejie, et al. "Etazolate abrogates the lipopolysaccharide (LPS)-induced downregulation of the cAMP/pCREB/BDNF signaling, neuroinflammatory response and depressive-like behavior in mice." Neuroscience 263 (2014): 1-14. PMID24434771		Western Blot; *Mouse*
Dong, Cun-Jian, et al. "Presynaptic Inhibition by α2 Receptor/Adenylate Cyclase/PDE4 Complex at Retinal Rod Bipolar Synapse." The Journal of Neuroscience 34.28 (2014): 9432-9440. PMID25009274		Immunohistochemistry; *Rat*
Zhai, Kui, et al. "Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms." Cellular signalling 26.5 (2014): 1001-1010. PMID24463006		Western Blot; *Rat*
Salazar, Norma C et al. “GRK2 Blockade with βARKct Is Essential for Cardiac Β2-Adrenergic Receptor Signaling Towards Increased Contractility.” Cell Communication and Signaling : CCS 11 (2013): 64. PMC3846709		Immunoprecipitation, Western Blot; *Mouse*
Al-Tawashi, Azza, and Chris Gehring. “Phosphodiesterase Activity Is Regulated by CC2D1A That Is Implicated in Non-Syndromic Intellectual Disability.” Cell Communication and Signaling?: CCS 11 (2013): 47. PMC3704924		Immunocytochemistry, Western Blot; *Mouse*
Wang, Zhen-Zhen et al. “RNA Interference-Mediated Phosphodiesterase 4D Splice Variants Knock-down in the Prefrontal Cortex Produces Antidepressant-Like and Cognition-Enhancing Effects.” British Journal of Pharmacology 168.4 (2013): 1001–1014. PMC3631387		Western Blot; *Mouse*
Michalski, Joel, et al. "Attenuation of inhibitory prostaglandin E2 signaling in Human lung fibroblasts is mediated by phosphodiesterase 4." American journal of respiratory cell and molecular biology 47.6 (2012): 729-737. PMID23043089		Western Blot; *Human*
Al-Tawashi, Azza et al. “Protein Implicated in Nonsyndromic Mental Retardation Regulates Protein Kinase A (PKA) Activity.” The Journal of Biological Chemistry 287.18 (2012): 14644–14658. PMC3340277		Immunohistochemistry, Western Blot; *Mouse*
Keravis, Thérèse et al. “Disease Progression in MRL/lpr Lupus-Prone Mice Is Reduced by NCS 613, a Specific Cyclic Nucleotide Phosphodiesterase Type 4 (PDE4) Inhibitor.” Ed. Pierre Bobé. PLoS ONE 7.1 (2012): e28899. PMC3256138		Western Blot; *Mouse*