Phospho-JAK3 Blocking Peptide

General Information

Product

Phospho-JAK3 Peptide

Description

Antigenic Blocking Peptide Phosphorylated Tyrosine-protein kinase JAK3 Antibody

Verified Applications

Immunodepletion, Immunocompetition

Peptide Description

Synthetic peptide taken within amino acid region 750-800 on human JAK3 protein. Phosphorylated tyrosine 785 residue.

Physical Properties

Quantity

250 µg

Volume

100 µl

Form

Antigenic Blocking Peptide

Storage

-20⁰C for long term storage

Application Protocol

Competition/Depletion assay

Refer to competition assay protocol

Protein

Uniprot #

P52333

Overview

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Endomembrane system; Peripheral membrane protein; Cytoplasm

Expression

In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

Structure

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Alternative Nomenclature

JAK 3 

JAK L 

Janus kinase 3 (a protein tyrosine kinase, leukocyte) 

Janus kinase 3 

L-JAK 

Leukocyte janus kinase 

Protein tyrosine kinase leukocyte 

Tyrosine protein kinase JAK3