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General Information
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Product
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Phospho-PDE4A PKA site Antibody BIOTIN-Conjugated
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Description
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BIOTIN-Conjugated Phosphorylated Phosphodiesterase 4A Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine residue.
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| Specificity |
This antibody detects all known long-form variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phosphorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with other members of the PDE family including PDE4B or PDE4D. |
| Note |
This antibody is ideal for studying phosphorylation dependent signal transduction research of PDE4 activation |
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.57 µg/µl in antibody stabilization buffer
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Immunoprecipitation
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1:150
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Western Blot
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1:500
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Protein
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Overview
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Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
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Molecular Function
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Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
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Subcellular Location
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Apical cell membrane, Cytoplasm, Membrane
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Expression
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Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
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Structure
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Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).
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Alternative Nomenclature
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cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
DKFZp686M11213 antibody
5''-cyclic phosphodiesterase 4D antibody
cAMP specific 3' 5' cyclic phosphodiesterase 4D antibody
cAMP specific phosphodiesterase 4D antibody
cAMP specific phosphodiesterase PDE4D6 antibody
cAMP-specific 3'' antibody
cyclic AMP phosphodiesterase antibody
DPDE 3 antibody
FLJ97311 antibody
HSPDE4D antibody
PDE 43 antibody
Pde4d antibody
PDE4DN2 antibody
Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
Phosphodiesterase 4D cAMP specific
Phosphodiesterase E3 dunce homolog Drosophila antibody
STRK 1 antibody
STRK1 antibody
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Phospho-PDE4A PKA site Blocking Peptide
Phospho-PDE4A Antibody
Phospho-PDE4A Antibody FITC
Phospho-PDE4A PKA site Positive Control
