Phospho-PDE7A Antibody BIOTIN

General Information

Product

Phospho-PDE7A Antibody BIOTIN-Conjugated

Description

BIOTIN-Conjugated High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated Synthetic cyclic peptide common to all PDE7A variants

Specificity

This antibody is specific for PDE7A variants and will not cross-react with PDE7B or other PDE family members.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

BIOTIN-Conjugated

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P70453

Overview

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.

Molecular Function

Hydrolase, 3', 5' -cyclic-AMP phosphodiesterase activity

Subcellular Location

Cytoplasm

Expression

PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart. Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle.

Structure

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Alternative Nomenclature

HCP 1 antibody

P2A antibody

PDE 7 antibody

PDE 7A antibody

PDE1/PDE2 yeast human complement of antibody

PDE7 antibody

PDE71 antibody

Phosphodiesterase 7A antibody

Phosphodiesterase isozyme 7 antibody

Rolipram insensitive phosphodiesterase type 7 antibody

TM22 antibody