S100 beta Antibody

General Information

Product

S100 beta Antibody Affinity Purified

Description

Affinity Purified Protein S100-P Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

C-epitope synthetic peptide corresponding to unique amino acid sequences on human S100 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P25815

Overview

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).

Molecular Function

Calcium-dependent protein binding, calcium ion binding, magnesium ion binding

Subcellular Location

Nucleus, cytoplasm, Cell projection > microvillus membrane

Expression

Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial n

Structure

Homodimer and heterodimer with S100A1. Interacts with S100PBP and S100Z. Interacts with CACYBP in a calcium-dependent manner. Dimeric form binds to and activates EZR/Ezrin by unmasking its F-actin binding sites. Interacts with PPP5C (via TPR repeats); the

Alternative Nomenclature

NEF antibody

S 100 calcium binding protein beta chain antibody

S 100 protein beta chain antibody

S 100 protein beta subunit antibody

S100 calcium-binding protein B antibody