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General Information
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Product
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TdT Antibody BIOTI
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Description
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BIOTIN-Conjugated DNA nucleotidylexotransferase isoform 2 Antibody C-epitope
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Verified Applications
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CM, ELISA, ICC, IF, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Chicken, Human, Monkey, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 450-500 on human Terminal deoxynucleotidyltransferase protein.
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Accession #
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NCBI: NP_004079
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.6 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Determinant
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C-epitope
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Storage
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-20⁰C for long term storage
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Application Protocol
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Confocal Microscopy
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1:250
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Dot Blot
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1:10,000
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ELISA
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1:10,000
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Immunocytochemistry
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1:250
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Immunofluorescence
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1:250
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Immunohistochemistry
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1:250
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Immunoprecipitation
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1:250
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Western Blot
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1:1,000
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Protein
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Uniprot #
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P04053
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Overview
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Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
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Molecular Function
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Nucleotidyltransferase, Transferase, DNA binding
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Subcellular Location
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Nucleus
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Structure
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Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.
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Alternative Nomenclature
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Deoxynucleotidyltransferase terminal antibody
DNA nucleotidylexotransferase antibody
DNTT antibody
Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
TDT antibody
Terminal addition enzyme antibody
Terminal deoxynucleotidyltransferase antibody
Terminal transferase antibody
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TdT Blocking Peptide
TdT Antibody
TdT Antibody FITC
TdT Positive Control
