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General Information
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Product
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CX3CL1 Antibody BIOTIN
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Description
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BIOTIN-Conjugated Fractalkine Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Monkey, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid reigon 350-395 on mouse Fractalkine protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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BIOTIN-Conjugated
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:10,000
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Immunoprecipitation
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1:150
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Western Blot
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1:500
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Protein
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Uniprot #
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O35188
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Overview
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Acts as a ligand for both CX3CL1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9177350, PubMed:10382755).
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Molecular Function
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CCR chemokine receptor binding, chemokine activity, CX3C chemokine receptor binding, integrin binding
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Subcellular Location
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Cell membrane, Single-pass type I membrane protein, Processed fractalkine : Secreted
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Expression
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Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.
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Structure
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Signal, Transmembrane, Transmembrane helix
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Alternative Nomenclature
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A 152E5.2 antibody
AB030188 antibody
ABCD3 antibody
AI848747 antibody
C-X3-C motif chemokine 1 antibody
C3Xkine antibody
Chemokine C X3 C motif ligand 1 antibody
CX3C membrane anchored chemokine antibody
Cx3cl1 antibody
CXC3 antibody
CXC3C antibody
D8Bwg0439e antibody
FKN antibody
Fractalkine antibody
Neurotactin antibody
NTN antibody
NTT antibody
Processed fractalkine antibody
SCYD 1 antibody
Small inducible cytokine D1 antibody
X3CL1_HUMAN antibody
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CX3CL1 Antibody
CX3CL1 Antibody FITC
CX3CL1 Blocking Peptide
CX3CL1 Positive Control
