PRAS40 Positive Control

General Information

Product

PRAS40 Positive Control

Description

Western Blot Positive Control Proline-rich AKT1 substrate 1 isoform a Antibody

Verified Applications

WB

Immunogen

Purified protein

Accession #

NCBI:  NP_115751

Physical Properties

Quantity

5 Applications

Volume

Inquire

Form

Western Blot Positive Control

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q96B36

Overview

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Molecular Function

negative regulation of cell size, negative regulation of protein kinase activity, negative regulation of TOR signaling, neurotrophin TRK receptor signaling pathway, regulation of cellular response to heat, regulation of neuron apoptotic process

Subcellular Location

Cytoplasm > cytosol

Expression

Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).

Alternative Nomenclature

40 kDa proline rich AKT substrate antibody
AKT1 S1 antibody
AKT1 substrate 1 (proline rich) antibody
AKT1S1 antibody
Lobe antibody
PRAS antibody
PRAS40 antibody
Proline rich Akt substrate 40 kDa antibody
Proline-rich AKT1 substrate 1 antibody