StripOBuffer Western Blot Stripping Buffer

Product no.: FGI-1989

$195.00

Buffer Size

Overview
Product	StripOBufferTM
Description	StripOBufferTM is a specially formulated water based buffer that will strip antibodies from antigens in 5-10 minutes at room temperature
Volume	250 ML
Concentration	Working solution
Storage	Room temperature for short-term storage
Applications	25 mL of StripObuffer can be used on 2 mini-blots or 1 large blot.

Advantages

Save time by re-using blots
Preserve protein and antigen samples
Eliminate issues with proper equal protein loading
Compare phosphorylated and non-phosphorylated state of the same protein
on the same blot
Reconfirm protein expression by re-probing the same blot with different set of
antibodies raised against antibodies from different vendors or same or different epitopes
Preform multiple stripping’s while maintaining antigenicity
No heating required
No β-mercaptoethanol required – No smell
Compatible with pure Nitrocellulose or Nylon supported Nitrocellulose membranes

Protocol

Shake the reagent to dissolve any precipitated material prior to use. Long-term storage at 4oC may develpp some precipitate.
Rinse the membrane in distilled water first and then in stripping buffer at least once.
Immerse the membrane completely in 25 mL of stripping buffer for 2 mini gels (10 X 10 cm) or one large gel membrane.
Gently shake the membrane on a rocker for 30-45 minutes at room temperature.
Rinse the membrane with distilled water. Wash the membranes 3 times in excess water to remove the stripObuffer completly.
Rinse the membrane with TBST or the buffer in which your primary antibody is diluted.
Proceed for blocking stage.

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FGI-1943 SolObuffer Cell Lysis Kit $175.00 *	FGI-1933 StainODye Nitrocellulose Membrane Protein Stain $150.00 *

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