StripOBuffer Western Blot Stripping Buffer

Product no.: FGI-1989

$195.00



Overview

 

 

Product

 

StripOBufferTM

Description

StripOBufferTM is a specially formulated water based buffer that will strip antibodies from antigens in 5-10 minutes at room temperature

Volume

250 ML

Concentration

Working solution

Storage

Room temperature for short-term storage

Applications

25 mL of StripObuffer can be used on 2 mini-blots or 1 large blot.

 

Advantages

 

 

  • Save time by re-using blots
  • Preserve protein and antigen samples
  • Eliminate issues with proper equal protein loading
  • Compare phosphorylated and non-phosphorylated state of the same protein
    on the same blot
  • Reconfirm protein expression by re-probing the same blot with different set of
    antibodies raised against antibodies from different vendors or same or different epitopes
  • Preform multiple stripping’s while maintaining antigenicity
  • No heating required
  • No β-mercaptoethanol required – No smell
  • Compatible with pure Nitrocellulose or Nylon supported Nitrocellulose membranes

Protocol

 

 

  1. Shake the reagent to dissolve any precipitated material prior to use. Long-term storage at 4oC may develpp some precipitate.
  2. Rinse the membrane in distilled water first and then in stripping buffer at least once. 
  3. Immerse the membrane completely in 25 mL of stripping buffer for 2 mini gels (10 X 10 cm) or one large gel membrane.
  4. Gently shake the membrane on a rocker for 30-45 minutes at room temperature.
  5. Rinse the membrane with distilled water. Wash the membranes 3 times in excess water to remove the stripObuffer completly.
  6. Rinse the membrane with TBST or the buffer in which your primary antibody is diluted.
  7. Proceed for blocking stage.

 

 

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