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General Information
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Product
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AGE Antibody
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Description
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Affinity Purified Advance Glycated Endproduct Antibody
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Verified Applications
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ELISA, WB
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Host
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Rabbit
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Species Cross Reactivity
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Bovine, Human, Monkey, Panda
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Immunogen
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Purified glycated ribonuclease corresponding to AGE. Glycated ribonuclease (RNase) prepared by incubating RNase with glucose at 37⁰C for several months.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.55 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Western Blot
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1:500
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Protein
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Overview
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Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators.
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Molecular Function
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Inflammatory Response
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Subcellular Location
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Isoform 1 : Cell membrane; Single-pass type I membrane protein
Isoform 2 : Secreted
Isoform 10 : Cell membrane 1 Publication; Single-pass type I membrane protein
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Expression
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Endothelial cells.
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Structure
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Interacts with S100A1 and APP (By similarity). Interacts with S100B, S100A12 and S100A14. Constitutive homodimer; disulfide-linked.
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Alternative Nomenclature
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Advanced glycation end products antibody
AGEs antibody
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AGE Antibody FITC
AGE Antibody BIOTIN
AGE Blocking Peptide
AGE Positive Control
