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General Information
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Product
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AMPK alpha 2 Antibody
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Description
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Affinity Purified 5'-AMP-activated protein kinase catalytic subunit alpha-2 Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 480-530 on rat 5'-AMP-activated protein kinase catalytic subunit alpha-2 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5-1.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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Affinity Purified
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:50,000
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| Immunohistochemistry |
1:50-1:250 |
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Immunoprecipitation
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1:200
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Western Blot
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1:2,500-1:5,000
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Protein
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Uniprot #
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Q09137
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Overview
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Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators.
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Molecular Function
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[acetyl-CoA carboxylase] kinase activity
[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity
AMP-activated protein kinase activity
ATP binding Source: RGD
chromatin binding
histone serine kinase activity
metal ion binding -KW
protein binding
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Subcellular Location
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Nucleus, Cytoplasm
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Expression
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Skeletal muscle, lower levels in liver, heart and kidney.
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Structure
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The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.
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Alternative Nomenclature
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5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody
ACACA kinase antibody
Acetyl-CoA carboxylase kinase antibody
AMPK alpha 2 chain antibody
AMPK subunit alpha-2 antibody
AMPK2 antibody
AMPKa2 antibody
HMGCR kinase antibody
Hydroxymethylglutaryl-CoA reductase kinase antibody
PRKAA antibody
PRKAA2 antibody
Protein kinase AMP activated alpha 2 catalytic subunit antibody
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AMPK alpha 2 Antibody BIOTIN
AMPK alpha 2 Antibody FITC
AMPK alpha 2 Blocking Peptide
AMPK alpha 2 Positive Control
