ARF1 Antibody

Product no.: ARF1-101AP

$320.00

General Information
Product	ARF1 Antibody
Description	Affinity Purified ADP-ribosylation factor 1 Antibody
Verified Applications	ELISA, ICC/IF, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desied antigenicity.
Specificity	does not cross react to other members of the Ras family or to other ARF members. This antibody will label ARF isoform 1, 2, 3, and 4.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.58-0.65 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:100
Immunocytochemistry	Use at an assay dependent concentration.
Western Blot	1:500. Predicted Molecular Weight 21 kDa
Protein
Uniprot #	P84079
Overview	GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.
Molecular Function	ADP-ribosyltransferase; GTP binding; ER-Golgi transport
Subcellular Location	Gogli apparatus
Expression	Cell junction; Membrane; Synapse
Structure	Belongs to the small GTPase superfamily, Arf family
Alternative Nomenclature	DJ133P16.1 antibody

Protein information supplied by UniProt

Fabgennix ARF1 Antibody has been used in 1 Publication

Lin, Lin et al. “The Antibiotic Gentamicin Inhibits Specific Protein Trafficking Functions of the Arf1/2 Family of GTPases.” Antimicrobial Agents and Chemotherapy 55.1 (2011): 246–254. PMC3019663

Western Blot; Rat

Publishing research using ARF1-101AP? Please let us know, we can cite the reference in this datasheet.

Customer ratings for ARF1 Antibody

Number of ratings: 2
Average rating: 4.5

Good
from Verified Customer on 12/11/2020

Immunoprecipitation preformed using rat whole brain tissue lysate. 1000 ug total protein input. 3.5 ul/mg lysate incubated for 17 hours at 4C. Able to get the antibody to work well for tissue lysate, but was unable to work in ell lysates.

Excellent
from Anonymous on 11/14/2015

Immunocytochemistry/Immunofluorescence
Human Cell (Neuroblastoma) Paraformaldehyde
Permeabilization-15 mins in 0.01% Triton X
Serum as blocking agent for 30 minutes
1:100 antibody dilution for 2 hours at 20C

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