B-raf Antibody


B-raf Antibody

Product no.: BRAF-101AP
$295.00
$265.00


General Information

 

 

Product

 

B-raf Antibody

Description

Affinity Purified Serine/threonine-protein kinase B-raf Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human serine/threonine-protein kinase B-raf protein.

Accession #

NCBINP_004324.2


Physical Properties

 

 

Quantity

 

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20C for long term storage


Application Protocol

 

ELISA

 

1:10,000

Immunohistochemistry 1:50-1:100

Immunoprecipitation

1:200

Western Blot

1:500. Molecular Weight: 84.4


Protein

 

Uniprot #

 

P15056

Overview

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Nucleus, Cytoplasm, Cell membrane

Expression

Brain and testis.

Structure

Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2.

Alternative Nomenclature

94 kDa B raf protein antibody

B raf 1 antibody

B Raf proto oncogene serine threonine protein kinase antibody

B RAF1 antibody

B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody

BRAF 1 antibody

cRmil antibody

Murine sarcoma viral v raf oncogene homolog B1 antibody

NS7 antibody

Oncogen BRAF antibody

p94 antibody

Proto-oncogene B-Raf antibody

Proto-oncogene c-Rmil antibody

RAFB1 antibody

RMIL antibody

Serine/threonine-protein kinase B-raf antibody

v-Raf murine sarcoma viral oncogene homolog B1 antibody

   

Protein information supplied by UniProt
 

 

Accessories

Product Note Status Price
B-raf Antibody BIOTIN B-raf Antibody BIOTIN
BRAF-BIOTIN $350.00 *
B-raf Antibody FITC B-raf Antibody FITC
BRAF-FITC $350.00 *
B-raf Blocking Peptide B-raf Blocking Peptide
P-BRAF $145.00 *
B-raf Positive Control B-raf Positive Control
PC-BRAF $185.00 *
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Browse these categories as well: Protein Kinases, Serine/Threonine Kinases, Apoptosis, Apoptosis, Oncoproteins, Cell Cycle, Cell Cycle, Cell Differentiation, Primaries