Dopamine Receptor D3 Antibody

General Information

Product

Dopamine Receptor D3 Antibody

Description

Affinity Purified D(3) dopamine receptor Antibody

Verified Applications

Dot Blot, ELISA, ICC/IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Dopamine Receptor D3 protein, peptide was covalently modified.

Sequence: CQACHVSPELYRATTWGY

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

DB

1:50,000

ELISA

1:50,000

Immunoprecipitation

Use at an assay dependent dilution. 2 µl of this antibody will immunoprecipitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells.

Western Blot

1:1,000-1:1,5000. Detects a band of approximately 50 kDa (predicted molecular weight 50 kDa).

Protein

Uniprot #

P19020

Overview

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation (By similarity).

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane, Multi-pass membrane protein

Expression

Mainly in limbic areas of brain.

Structure

Interacts with GRK4 and PALM (By similarity). Interacts with CLIC6.

Alternative Nomenclature

3dopamine receptor antibody

D antibody

D(3) dopamine receptor antibody

DRD3 antibody

ETM1 antibody

FET1 antibody