DNA PKcs Antibody

Product no.: DNPK1-101Y

$285.00

Description

General Information
Product	DNA PKcs Antibody
Description	Affinity Purified DNA-dependent protein kinase catalytic subunit Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Chicken
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 4000-4128 of human DNPK1 protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Immunoglobulin	IgY
Form	Affinity Purified
Purification Method	Immobilized antigen affinity chromatography
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunohistochemistry	1:50-1:150
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P78527
Overview	Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.
Molecular Function	Kinase, Serine/threonine-protein kinase, Transferase
Subcellular Location	Nucleus
Expression	Found in axons of the central and peripheral nervous system;differentiated cell types of the intestine
Structure	Belongs to the immunoglobulin superfamily. DCC family;Contains 6 fibronectin type-III domains;Contains 4 Ig-like C2-type (immunoglobulin-like) domains
Alternative Nomenclature	DNA dependent protein kinase catalytic subunit antibody DNA PK catalytic subunit antibody DNA-PKcs antibody DNAPK antibody DNPK1 antibody Hyper radiosensitivity of murine scid mutation, complementing 1 antibody HYRC antibody HYRC1 antibody p350 antibody p460 antibody PKRDC antibody Protein Kinase DNA Activated Catalytic Polypeptide antibody XRCC7 antibody

Protein information supplied by UniProt