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General Information
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Product
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DNA PKcs Antibody
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Description
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Affinity Purified DNA-dependent protein kinase catalytic subunit Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Chicken
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 4000-4128 of human DNPK1 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgY
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Form
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Affinity Purified
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:10,000
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| Immunohistochemistry |
1:50-1:150 |
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Immunoprecipitation
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1:200
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Western Blot
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1:500
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Protein
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Uniprot #
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P78527
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Overview
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Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.
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Molecular Function
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Kinase, Serine/threonine-protein kinase, Transferase
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Subcellular Location
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Nucleus
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Expression
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Found in axons of the central and peripheral nervous system;differentiated cell types of the intestine
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Structure
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Belongs to the immunoglobulin superfamily. DCC family;Contains 6 fibronectin type-III domains;Contains 4 Ig-like C2-type (immunoglobulin-like) domains
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Alternative Nomenclature
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DNA dependent protein kinase catalytic subunit antibody
DNA PK catalytic subunit antibody
DNA-PKcs antibody
DNAPK antibody
DNPK1 antibody
Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
HYRC antibody
HYRC1 antibody
p350 antibody
p460 antibody
PKRDC antibody
Protein Kinase DNA Activated Catalytic Polypeptide antibody
XRCC7 antibody
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DNA PKcs FITC
DNA PKcs Blocking Peptide
DNA PKcs Antibody BIOTIN
DNA PKcs Positive Control
