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General Information
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Product
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EAAT1 Antibody
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Description
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Affinity Purified Excitatory Amino Acid Transporter 1 Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Monkey, Mouse, Rat
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Immunogen
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Synthetic peptide corresponding to C terminal amino acids 480-543 of Mouse EAAT1 protein. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to carrier protein using heterobifunctional cross linker for immunogen preparation.
Sequence:
KPYQLIAQDNEPEKPVADSETK
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| Specificity |
Will not cross react with EAAT2 protein. |
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Accession #
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NCBI: NP_683740.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.61 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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| Immunohistochemistry |
1:100 |
| Immunoprecipitation |
1:200 |
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Western Blot
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1:500
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Protein
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Uniprot #
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P56564
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Overview
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Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
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Molecular Function
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high-affinity glutamate transmembrane transporter activity;sodium:dicarboxylate symporter activity
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Subcellular Location
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plasma membrane; cell surface; fibril;neuronal cell body
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Expression
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Purkinje cell layer in cerebellum,lung,spleen, skeletal muscle and testis.
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Structure
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Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily has a Transmembrane helix.
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Alternative Nomenclature
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EA6 antibody
EAAT1 antibody
Excitatory amino acid transporter 1 antibody
FLJ25094 antibody
GLAST antibody
Glial high affinity glutamate transporter antibody
glutamate/aspartate transporter, high affinity, sodium-dependent antibody
High affinity neuronal glutamate transporter antibody
Slc1a3 antibody
Sodium dependent glutamate/aspartate transporter antibody
Solute carrier family 1 member 3 antibody
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EAAT1 Antibody FITC
EAAT1 Antibody BIOTIN
EAAT1 Blocking Peptide
EAAT1 Positive Control
