FAK1 Antibody

Product no.: FAK1-101Y

$285.00

Description

General Information
Product	FAK1 Antibody
Description	Affinity Purified Focal Adhesion Kinase 1
Verified Applications	ELISA, IHC, IP, WB
Host	Chicken
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 740-790 on human focal adhesion kinase 1 protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.6 µg/µl in antibody stabilization buffer
Immunoglobulin	IgY
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunocytochemistry	1:100
Immunofluorescence	1:100
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q05397
Overview	Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
Molecular Function	Developmental protein; Kinase; Transferase; Tyrosine-protein kinase
Subcellular Location	Cell junction › focal adhesion Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm › cell cortex Cytoplasm › cytoskeleton Cytoplasm › cytoskeleton › microtubule organizing center › centrosome Nucleus
Expression	Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.
Structure	Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain.
Alternative Nomenclature	FADK 1 antibody FADK antibody FAK 1 antibody FAK related non kinase polypeptide antibody FAK1 antibody Focal adhesion kinase 1 antibody Focal adhesion kinase isoform FAK Del33 antibody Focal adhesion kinase related nonkinase antibody FRNK antibody p125FAK antibody pp125FAK antibody PPP1R71 antibody Protein phosphatase 1 regulatory subunit 71 antibody Protein tyrosine kinase 2 antibody Protein Tyrosine Kinase Cytoplasmic antibody PTK 2 antibody PTK2 protein tyrosine kinase 2 antibody

Protein information supplied by UniProt