StripOBuffer Western Blot Stripping Buffer

Product no.: FGI-1989

Overview

 

 

Product

 

StripOBufferTM

Description

StripOBufferTM is a specially formulated water based buffer that will strip antibodies from antigens in 5-10 minutes at room temperature

Volume

100 ML

Concentration

10X solution

Storage

Room temperature for short-term storage

Applications

25 mL of StripObuffer can be used on 25-50 mini-blots.

 

Advantages

 

 

  • Save time by re-using blots
  • Preserve protein and antigen samples
  • Eliminate issues with proper equal protein loading
  • Compare phosphorylated and non-phosphorylated state of the same protein
    on the same blot
  • Reconfirm protein expression by re-probing the same blot with different set of
    antibodies raised against antibodies from different vendors or same or different epitopes
  • Preform multiple stripping’s while maintaining antigenicity
  • No heating required
  • No β-mercaptoethanol required – No smell
  • Compatible with Nitrocellulose and PVDF membranes

Protocol

 

 

  1. Dilute (1:10) the supplied buffer in distilled water prior to use.
  2. Rinse the membrane in distilled water first and then in stripping buffer at least once. 
    Multiple rinses with buffer results in clearer bands on autoradiograms
  3. Immerse the membrane completely in 10 mL of stripping buffer for each
    mini-gel membrane.
  4. Gently shake the membrane on a rocker for 5-10 minutes at room temperature.
  5. Rinse the membrane with distilled water.
  6. Rinse the membrane with TBST or the buffer in which your primary antibody is diluted.
  7. Proceed for blocking stage.

 

 

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