Histone H3 Antibody BIOTIN

General Information

Product

Histone H3 Antibody BIOTIN-Conjugated

Description

BIOTIN-Conjugated Histone H3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, S. cerevisiae

Immunogen

Synthetic peptide taken within amino acid 1-50 on Sacchoromyces cerevisiae protein HH3 protein.

Accession #

Gen Bank: EDZ69636.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

BIOTIN-Conjugated

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

B5VR08

Overview

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones. Can bind to DNA (in vitro).

Molecular Function

Chromatin regulator, Methyltransferase, Transferase

Subcellular Location

Nucleus

Structure

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

Contains 1 DOT1 domain.

Alternative Nomenclature

H3 histone family, member J antibody

H3 histone antibody

H3 histone family, member A antibody

H3 histone family, member B antibody

H3 histone family, member C antibody

H3 histone family, member D antibody

H3 histone family, member F antibody

H3 histone family, member H antibody

H3 histone family, member I antibody

H3 histone family, member K antibody

H3 histone family, member L antibody

H3 histone, family 3A antibody

H3.3A antibody

H3F1K antibody

H3F3 antibody

Histone cluster 1, H3a antibody

Histone cluster 1, H3b antibody

Histone cluster 1, H3c antibody

Histone cluster 1, H3d antibody

Histone cluster 1, H3e antibody

Histone cluster 1, H3f antibody

Histone cluster 1, H3g antibody

Histone cluster 1, H3i antibody

Histone cluster 1, H3j antibody