Insulin Receptor Antibody

Product no.: INSR1-112AP

$285.00

Description

General Information
Product	Insulin Receptor Antibody
Description	Affinity Purified Insulin receptor alpha and beta Antibody Extreme C-epitope
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 950-1000 on human Insulin receptor, transcript variant 2 protein.
Accession #	NCBI: NM_001079817.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55-0.75 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Purification Method	Immobilized antigen affinity chromatography
Clonality	Polyclonal
Determinant	Extreme C-epitope
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunocytochemistry	1:100
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P06213
Overview	Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2.
Molecular Function	Kinase, Receptor, Transferase, Tyrosine-protein kinase
Subcellular Location	Cell membrane; Single-pass type I membrane protein
Expression	Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.
Structure	Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds.
Alternative Nomenclature	CD220 antibody HHF5 antibody HIR A antibody INSR alpha antibody Insulin receptor antibody Insulin receptor subunit alpha antibody IR antibody HHF5 antibody human insulin receptor antibody Insulin receptor subunit beta antibody IR 1 antibody

Protein information supplied by UniProt.