IRE1A Antibody

Product no.: IRE1A-101Y

$285.00

Description

General Information
Product	IRE1A Antibody
Description	Affinity Purified Serine/Threonine-protein kinase/endoribonuclease IRE1 Antibody
Verified Applications	ELISA, IP, WB
Host	Chicken
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-977 on human IRE1A protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.59-0.68 µg/µl in antibody stabilization buffer
Immunoglobulin	IgY
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:50-1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	O75460
Overview	Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Molecular Function	Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase
Subcellular Location	Endoplasmic reticulum membrane; Single-pass type 1 membrane protein
Expression	Ubiquitously expressed. High levels observed in pancreatic tissue.
Structure	Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds, HSPA5, a negative regulator of the unfolded protein response.
Alternative Nomenclature	Endoplasmic reticulum (ER) to nucleus signalling 1 antibody Endoribonuclease antibody ER to nucleus signaling 1 antibody ERN 1 antibody hIRE 1p antibody Inositol requiring 1 antibody Inositol requiring protein 1 antibody inositol-requiring enzyme 1 antibody IRE 1 antibody IRE 1a antibody IRE 1P antibody Ire1 alpha antibody MGC163277 antibody MGC163279 antibody Protein kinase/endoribonuclease antibody RGD1559716 antibody Serine/threonine protein kinase/endoribonuclease IRE1 antibody

Protein information supplied by UniProt