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General Information
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Product
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IRE1A Antibody
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Description
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Affinity Purified Serine/Threonine-protein kinase/endoribonuclease IRE1 Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Chicken
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-977 on human IRE1A protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.59-0.68 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgY
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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| Immunohistochemistry |
1:50-1:100 |
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Immunoprecipitation
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1:200
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Western Blot
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1:500
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Protein
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Uniprot #
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O75460
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Overview
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Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
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Molecular Function
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Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase
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Subcellular Location
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Endoplasmic reticulum membrane; Single-pass type 1 membrane protein
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Expression
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Ubiquitously expressed. High levels observed in pancreatic tissue.
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Structure
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Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds, HSPA5, a negative regulator of the unfolded protein response.
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Alternative Nomenclature
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Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
Endoribonuclease antibody
ER to nucleus signaling 1 antibody
ERN 1 antibody
hIRE 1p antibody
Inositol requiring 1 antibody
Inositol requiring protein 1 antibody
inositol-requiring enzyme 1 antibody
IRE 1 antibody
IRE 1a antibody
IRE 1P antibody
Ire1 alpha antibody
MGC163277 antibody
MGC163279 antibody
Protein kinase/endoribonuclease antibody
RGD1559716 antibody
Serine/threonine protein kinase/endoribonuclease IRE1 antibody
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IRE1A Antibody FITC
IRE1A Antibody BIOTIN
IRE1A Blocking Peptide
IRE1A Positive Control
