NHERF-1 Antibody

General Information

Product

NHERF-1 Antibody

Description

Affinity Purified Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 255-305 on human Na(+)/H(+) exchange regulatory cofactor NHE-RF1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

O14745

Overview

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.

Molecular Function

beta-2 adrenergic receptor binding

beta-catenin binding

chloride channel regulator activity

dopamine receptor binding

gamma-aminobutyric acid transmembrane transporter activity

growth factor receptor binding

myosin II binding

PDZ domain binding

pho

Subcellular Location

Plasma membrane, cytoplasm

Expression

Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

Alternative Nomenclature

EBP 50 antibody

Ezrin radixin moesin binding phosphoprotein 50 antibody

Na(+)/H(+) exchange regulatory cofactor NHE RF antibody

Na+/H+ exchange regulatory co factor antibody

NHERF 1 antibody

NHERF antibody

NHRF1_HUMAN antibody

NPHLOP2 antibody

Regulatory cofactor of Na(+)/H(+) exchanger antibody

Slc9a3r1 antibody

Sodium hydrogen exchanger regulatory factor 1 antibody

Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody

Solute carrier family 9 isoform 3 regulatory factor 1 antibody