RIPK2 Blocking Peptide

Product no.: P-RIPK2
$155.00


General Information

 

 

Product

 

RIPK2 Peptide

Description

Antigenic Blocking Peptide Receptor-interacting Serine/Threonine-protein kinase 2 Antibody

Verified Applications

Immunodepletion, Immunocompetition

Peptide Description

Synthetic Peptide

Accession #

GenBank: AAQ89173.1


Physical Properties

 

 

Quantity

 

250 µg

Volume

100 µl

Form

Antigenic Blocking Peptide

Storage

-20C for long term storage


Application Protocol

Competition/Depletion assay

Refer to competition assay protocol


Protein

 

Uniprot #

 

O43353

Overview

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Molecular Function

ATP binding; CARD domain binding; LIM domain binding; non-membrane spanning protein tyrosine kinase activity; Protein homodimerization activity; protein serine/threonine kinase activity; signal transducer activity

Subcellular Location

Cytoplasm

Expression

Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Structure

Found in a signaling complex consisting of at least ARHGEF2, NOD2 and RIPK2. Interacts with ARHGEF2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Binds to CFLAR/CLARP and CASP1 via their CARD domains. Binds to BIRC3/c-IAP1 and BIRC2/c-IAP2, TRAF1, TRAF2, TRAF5 and TRAF6. May be a component of both the TNFRSF1A and TNRFSF5/CD40 receptor complex. Interacts with NOD1. Interacts (via CARD domain) with NOD2 (via CARD domain). Interacts with MAP3K4; this interaction sequesters RIPK2 from the NOD2 signaling pathway. Interacts with IKBKG/NEMO. The polyubiquitinated protein interacts with MAP3K7/TAK1. Interacts with XIAP/BIRC4. Interacts with NLRP10. Interacts with CARD9.

Alternative Nomenclature

CARD carrying kinase antibody

CARD CONTAINING ICE ASSOCIATED KINASE antibody

CARD containing interleukin 1 beta converting enzyme associated kinase antibody

CARDIAK antibody

CLARP kinase antibody

GIG 30 antibody

Growth inhibiting gene 30 antibody

Receptor interacting protein 2 antibody

Receptor interacting serine threonine kinase 2

RICK antibody

RIP-like-interacting CLARP kinase antibody

   

Protein information supplied by UniProt
 

 

Accessories

Product Note Status Price
RIPK2 Antibody RIPK2 Antibody
RIPK2-201AP $285.00 *
RIPK2 Antibody FITC RIPK2 Antibody FITC
RIPK2-FITC $375.00 *
RIPK2 Antibody BIOTIN RIPK2 Antibody BIOTIN
RIPK2-BIOTIN $375.00 *
RIPK2 Positive Control RIPK2 Positive Control
PC-RIPK2 $200.00 *
Display accessory details