Smad4 Blocking Peptide

Product no.: P-SMAD4.401
$155.00


General Information

 

 

Product

 

Smad4 Peptide

Description

Antigenic Blocking Peptide Mothers against decapentaplegic homolog 4 N-epitope

Verified Applications

Immunocompetition, Immunodepletion

Peptide Description

Synthetic peptide taken within amino acid region 131-181 on human SMAD4 protein.

Accession #

NCBINP_005350.1


Physical Properties

 

 

Quantity

 

250 µg

Volume

100 µl

Form

Antigenic Blocking Peptide

Determinant

N-epitope

Storage

-20⁰C for long term storage


Application Protocol

Competition/Depletion assay

Refer to competition assay protocol


Protein

 

Uniprot #

 

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
hSMAD4 antibody
JIP antibody
MAD homolog 4 antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
OTTHUMP00000163548 antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody
SMAD4 antibody

   

Protein information supplied by UniProt

Accessories

Product Note Status Price
Smad4 Antibody Smad4 Antibody
SMAD4-401AP $285.00 *
Smad4 Antibody FITC Smad4 Antibody FITC
SMAD4.401-FITC $375.00 *
Smad4 Antibody BIOTIN Smad4 Antibody BIOTIN
SMAD4.401-BIOTIN $375.00 *
Smad4 Antibody Positve Control Smad4 Antibody Positve Control
PC-SMAD4 $200.00 *
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