Phospho-Cofilin Antibody

General Information

Product

Phospho-Cofilin Antibody

Description

Affinity Purified Phosphorylated Cofilin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 1-50 on human Phosphorylated Cofilin.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P23528

Overview

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity).

Molecular Function

actin filament binding

Subcellular Location

Plasma membrane, Cytoskeleton, Nucleus

Expression

Widely distributed in various tissues.

Structure

Belongs to the actin-binding proteins ADF family.

Alternative Nomenclature

18 kDa phosphoprotein antibody

CFL 1 antibody

CFL antibody

CFL1 antibody

COF1_HUMAN antibody

Cofilin 1 antibody

Cofilin 1 non muscle antibody

Cofilin antibody

Cofilin non muscle isoform antibody

Cofilin-1 antibody

epididymis secretory protein Li 15 antibody

HEL-S-15 antibody

non-muscle isoform antibody

p18 antibody