PDE1C Antibody

Product no.: PD1C-301AP

$320.00

General Information
Product	PDE1C Antibody
Description	Affinity Purified Human 3',5' cyclic nucleotide phosphodiesterase (HSPDE1C1A) Antibody N-epitope
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence on PDE1C gene. The sequence was taken within amino acid region 1-50.
Specificity	This antibody does not cross-react with PDE1A, PDE1B, or other PDE family members. This antibody will label all PDE1C variants including PDE1C1, PDE1C2, and PDE1C3.
Accession #	GenBank: U40371.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	N-epitope
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:40-1:100
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:40-1:100
Immunofluorescence	1:100
Immunohistochemistry	1:40-1:100
Immunoprecipitation	1:200
Western Blot	1:500-1:2,000
Protein
Uniprot #	Q14123
Overview	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP.
Molecular Function	Hydrolase, calmodulin-dependent cyclic-nucleotide phosphodiesterase activity, metal ion binding
Subcellular Location	Cytosol
Expression	Expressed in several tissues, including brain and heart.
Structure	Homodimer
Alternative Nomenclature	5''-cyclic nucleotide phosphodiesterase 1C Calcium/calmodulin dependent 3'5' cyclic nucleotide phosphodiesterase 1C Cam PDE 1C HCAM3 HSPDE1C1A Human 3'5' cyclic nucleotide Phosphodiesterase PDE 1C Phosphodiesterase 1C Phosphodiesterase 1C calmodulin dependent

Protein information supplied by UniProt

FabGennix PDE1C Antibody has been used in 16 Publications

Applications: Confocal microscopy, Immunodepletion, Immunofluorescence, Immunohistochemistry, and Western blot

Reactivity: Chicken, Human, Mouse, and Rat

Cesarini, Valeriana, et al. "Type 5 phosphodiesterase regulates glioblastoma multiforme aggressiveness and clinical outcome." Oncotarget (2017).

PMID: 28099939

Applications: Western Blot

Reactivity: Human

Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015).

PMID: 25578602

Applications: Immunohistochemistry

Reactivity: Human

Yougbaré, Issaka, et al. "Cyclic GMP catabolism up-regulation in MRL/lpr lupus-prone mice is associated with organ remodeling." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1842.7 (2014).

PMID: 24631654

Applications: Immunohistochemistry, Western Blot

Reactivity: Mouse

Guibinga, Ghiabe-Henri, Fiona Murray, and Nikki Barron. “HPRT-Deficiency Dysregulates cAMP-PKA Signaling and Phosphodiesterase 10A Expression: Mechanistic Insight and Potential Target for Lesch-Nyhan Disease?” Ed. Kjetil Tasken. PLoS ONE 8.5 (2013).

PMCID: 3653951

Applications: Western Blot

Reactivity: Human

Genders, Amanda J., et al. "cGMP phosphodiesterase inhibition improves the vascular and metabolic actions of insulin in skeletal muscle." American Journal of Physiology-Endocrinology and Metabolism 301.2 (2011).

PMID: 21653225

Applications: Immunofluorescence, Western Blot

Reactivity: Mouse

Abusnina, Abdurazzag, et al. "Down-regulation of cyclic nucleotide phosphodiesterase PDE1A is the key event of p73 and UHRF1 deregulation in thymoquinone-induced acute lymphoblastic leukemia cell apoptosis." Cellular signalling 23.1 (2011).

PMID: 20807569

Applications: Western Blot

Reactivity: Human

Abusnina, Abdurazzag, et al. "Anti‐proliferative effect of curcumin on melanoma cells is mediated by PDE1A inhibition that regulates the epigenetic integrator UHRF1." Molecular nutrition & food research 55.11 (2011).

PMID: 22045655

Applications: Western Blot

Reactivity: Human

Dong, Hongli, et al. "Inhibition of PDE3, PDE4 and PDE7 potentiates glucocorticoid-induced apoptosis and overcomes glucocorticoid resistance in CEM T leukemic cells." Biochemical pharmacology 79.3 (2010).

PMID: 19737543

Applications: Western Blot

Reactivity: Human

Ortiz-Capisano, M. Cecilia et al. “Calcium-Dependent Phosphodiesterase 1C Inhibits Renin Release from Isolated Juxtaglomerular Cells.” American Journal of Physiology - Regulatory, IntegRative and CompaRative Physiology 297.5 (2009).

PMCID: 2777770

Applications: Confocal microscopy, Immunohistochemistry, Immunofluorescence, Western Blot

Reactivity: Mouse

Tahseldar-Roumieh, Rima et al. “PDEs1-5 Activity and Expression in Tissues of Cirrhotic Rats Reveal a Role for Aortic PDE3 in NO Desensitization.” International Journal of Experimental Pathology 90.6 (2009).

PMCID: 2803251

Applications: Western Blot

Reactivity: Rat

Deplano, Stefania, et al. "Gene expression and protein localization of calmodulin‐dependent phosphodiesterase during ontogenesis of chick retina." Journal of neuroscience research 86.5 (2008).

PMID: 18041092

Applications: Immunohistochemistry, Western Blot

Reactivity: Chicken

Dunkern, Torsten R., and Armin Hatzelmann. "Characterization Of Inhibitors Of Phosphodiesterase 1C On A Human Cellular System." FEBS Journal 274.18 (2007).

PMID: 17697115

Applications: Western Blot

Reactivity: Human

Murray, Fiona, et al. "Expression and activity of cAMP phosphodiesterase isoforms in pulmonary artery smooth muscle cells from patients with pulmonary hypertension: role for PDE1." American Journal of Physiology-Lung Cellular and Molecular Physiology 292.1 (2007).

PMID: 16980375

Applications: Western Blot

Reactivity: Human

Santone, Rocco, et al. "Gene expression and protein localization of calmodulin‐dependent phosphodiesterase in adult Rat retina." Journal of neuroscience research 84.5 (2006).

PMID: 16881052

Applications: Confocal microscopy, Immunohistochemistry, Western Blot

Reactivity: Rat

Dolci, Susanna, et al. "Subcellular localization and regulation of type-1C and type-5 phosphodiesterases." Biochemical and biophysical research communications 341.3 (2006).

PMID: 16455054

Applications: Immunodepletion, Immunofluorescence, Western Blot

Reactivity: Human

Baxendale, Rhona W., and Lynn R. Fraser. "Mammalian sperm phosphodiesterases and their involvement in receptor‐mediated cell signaling important for capacitation." Molecular reproduction and development 71.4 (2005).

PMID: 15856425

Applications: Immunofluorescence, Western Blot

Reactivity: Mouse

Publishing research using PD1C-301AP? Please let us know, we can cite the reference in this datasheet.

On your PDE1c western blot data sheet there are multiple bands from different tissue origin lysates. As we are having problems with the size of PDE1c, can you tell us or mark these sizes of the PDE1C splice variants?

The PDE1C splice variants are described in detail in Yan C et al. The calmodulin-dependent phosphodiesterase gene PDE1C encodes several functionally different splice variants in a tissue-specific manner. J Biol Chem 271:25699-706 (1996). Please refer to this reference as it is useful in describing th