PDE3B Antibody

Product no.: PD3B-101AP

$320.00

General Information
Product	PDE3B Antibody
Description	Affinity Purified cGMP-inhibited 3',5'-cyclic phosphodiesterase B Antibody
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Chicken, Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 1060-1110 on human PDE3B protein.
Specificity	This antibody does not cross react with PDE4A variants nor with any other PDE famly members. This antibody will recognize PDE3B variants.
Accession #	GenBank: BAA09306
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.70 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
Dot Blot	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q13370
Overview	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Regulates cAMP binding of RAPGEF3.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Subcellular Location	Membrane; Multipass membrane protein
Expression	Abundant in adipose tissues.
Structure	Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6.
Alternative Nomenclature	5''-cyclic phosphodiesterase B antibody adipocyte cAMP phosphodiesterase cGMP inhibited antibody AI847709 antibody cAMP Phosphodiesterase, adipocyte, cGMP-inhibited antibody CGIP1 antibody CGIPDE B antibody cGMP inhibited 3 5 cyclic phosphodiesterase B antibody CGMP inhibited cAMP phophodiesterase antibody cGMP-inhibited 3'' antibody Cyclic GMP-inhibited phosphodiesterase B antibody Cyclic nucleotide phosphodiesterase antibody HcGIP1 antibody Phosphodiesterase 3B cGMP inhibited antibody

Protein information supplied by UniProt

Fabgennix PDE3B Antibody has been used in 4 Publications

Huang, Lei, et al. "Phosphoinositide 3-Kinase Gamma Contributes to Neuroinflammation in a Rat Model of Surgical Brain Injury." The Journal of Neuroscience 35.29 (2015): 10390-10401. PMC4510283

Western Blot; Rat

Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015). PMID25578602

Immunohistochemistry, Human

Dov, Avital, et al. "Diminished phosphodiesterase-8B potentiates biphasic insulin response to glucose." Endocrinology 149.2 (2008): 741-748. PMID17991719

Western Blot; Rat

Netherton, Stuart J., and Donald H. Maurice. "Vascular endothelial cell cyclic nucleotide phosphodiesterases and regulated cell migRation: implications in angiogenesis." Molecular pharmacology 67.1 (2005): 263-272. PMID15475573

Western Blot; Bovine, Human

Publishing research using PD3B-101AP? Please let us know, we can cite the reference in this datasheet.

Do you have any suggestions about the PDE3b antibody? It gives bands about 100 for different concentrations I used. I loaded 20 micrograms of cortex and cerebellum lysates and incubated the nitrocellulose membranes with the PDE1(a,b,c) and PDE3b antibodies.
1. Thank you for your inquiry about PD3B-101AP,
  I looked at the gel images you provided and am curious why you are using a high percentage or gradient gel for a protein that is above 100kDa. There are two issues that I can think of that may be affecting the results. The high percentage acrylamide may be reducing the efficiency of high MW proteins onto nitrocellulose membrane. The cortex is not the regions where we see PDE3B in high density, so it may be possible that the levels of PDE3B is already below the detection limit here. Second issue may be due to proteolysis. PDE3B is cleaved off by endogenous proteases if their activity is not checked properly. In order to curb this prepare the homogenates and samples in the presence of Fabgennix Protease Inhibitor Cocktail before the SDS-PAGE. Use PC-PDE3B to identify bands and validate the system.

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PDE3B Antibody

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