PDE4C Antibody

Product no.: PD4C-301AP

$320.00

Description
Publications

General Information
Product	PDE4C Antibody
Description	Affinity Purified cAMP-specific 3',5'-cyclic phosphodiesterase 4C Antibody
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic cyclic carboxy-terminal peptide common to all PDE4C variants.
Specificity	This antibody detects 3 isoforms of PDE4C subclasses: PDE4C1, PDE4C2, and PDE4C3.
Accession #	NCBI: NP_001074.2
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.65 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:200
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:200
Immunofluorescence	1:200
Immunohistochemistry	1:200
Immunoprecipitation	1:200
Western Blot	1:500-1:2,500
Protein
Uniprot #	Q08493
Overview	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Subcellular Location	Cell projection > cillium
Expression	Expressed in various tissues but not in cells of the immune system.
Structure	Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2.
Alternative Nomenclature	5''-cyclic phosphodiesterase 4C antibody cAMP specific 3' 5' cyclic phosphodiesterase 4C antibody cAMP-specific 3'' antibody DPDE 1 antibody MGC126222 antibody PDE 21 antibody PDE 4C antibody Pde4c antibody Phosphodiesterase 4C antibody Phosphodiesterase E1 dunce homolog antibody Phosphodiesterase type 4C antibody

Protein information supplied by UniProt

FabGennix PDE4C Antibody has been used in 15 Publications

Applications: Coimmunoprecipitation, Immunocytochemistry, Immunohistochemistry, and Western blot

Reactivity: Human, Mouse, and Rat

Cheng, Hao, et al. "siRNA‑mediated silencing of phosphodiesterase 4B expression affects the production of cytokines in endotoxin‑stimulated primary cultured microglia." Experimental and Therapeutic Medicine 12.4 (2016).

PMID: 27698721

Applications: Western Blot

Reactivity: Rat

Ji, Qing, et al. "Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation." Molecular medicine reports 13.2 (2016).

PMCID: 26706904

Applications: Western Blot

Reactivity: Rat

Massimi, Mara, et al. "Increase of Intracellular Cyclic AMP by PDE4 Inhibitors Affects HepG2 Cell Cycle Progression and Survival." Journal of Cellular Biochemistry (2016).

PMCID: 27859531

Applications: Western Blot

Reactivity: Human

Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015).

PMID: 25578602

Applications: Immunohistochemistry

Reactivity: Human

Yougbaré, Issaka, et al. "NCS 613 Exhibits Anti-Inflammatory Effects On Pbmcs From Lupus Patients By Inhibiting P38 MAPK And NF-Κb Signalling Pathways While Reducing Proinflammatory Cytokine Production." Canadian Journal Of Physiology & Pharmacology 91.5 (2013).

PMID: 23656347

Applications: Immunohistochemistry, Western Blot

Reactivity: Human

Haque, Mohammed Z., Paulo S. Caceres, and Pablo A. Ortiz. “β-Adrenergic Receptor Stimulation Increases Surface NKCC2 Expression in Rat Thick Ascending Limbs in a Process Inhibited by Phosphodiesterase 4.” American Journal of Physiology - Renal Physiology 303.9 (2012).

PMCID: 3518189

Applications: Western Blot

Reactivity: Rat

Keravis, Thérèse et al. “Disease Progression in MRL/lpr Lupus-Prone Mice Is Reduced by NCS 613, a Specific Cyclic Nucleotide Phosphodiesterase Type 4 (PDE4) Inhibitor.” Ed. Pierre Bobé. PLoS ONE 7.1 (2012).

PMCID: 3256138

Applications: Western Blot

Reactivity: Mouse

Abusnina, Abdurazzag, et al. "Down-regulation of cyclic nucleotide phosphodiesterase PDE1A is the key event of p73 and UHRF1 deregulation in thymoquinone-induced acute lymphoblastic leukemia cell apoptosis." Cellular signalling 23.1 (2011).

PMID: 20807569

Applications: Western Blot

Reactivity: Human

Yougbare, Issaka, et al. "NCS 613, a potent and specific PDE4 inhibitor, displays anti-inflammatory effects on Human lung tissues." American Journal of Physiology-Lung Cellular and Molecular Physiology 301.4 (2011).

PMID: 21784969

Applications: Immunocytochemistry, Western Blot

Reactivity: Human

Tahseldar-Roumieh, Rima et al. “PDEs1-5 Activity and Expression in Tissues of Cirrhotic Rats Reveal a Role for Aortic PDE3 in NO Desensitization.” International Journal of Experimental Pathology 90.6 (2009).

PMID: 2803251

Applications: Western Blot

Reactivity: Rat

Whitaker, Christopher M., and Nigel G. F. Cooper. “The Novel Distribution of Phosphodiesterase-4 Subtypes Within the Rat Retina.” Neuroscience 163.4 (2009).

PMCID: 2774823

Applications: Western Blot

Reactivity: Rat

Campos-Toimil, M et al. “Short-Term or Long-Term Treatments with a Phosphodiesterase-4 (PDE4) Inhibitor Result in Opposing Agonist-Induced Ca2+ Responses in Endothelial Cells.” British Journal of Pharmacology 154.1 (2008).

PMCID: 2438981

Applications: Western Blot

Reactivity: Human

Sachs, Benjamin D. et al. “P75 Neurotrophin Receptor Regulates Tissue Fibrosis through Inhibition of Plasminogen Activation via a PDE4/cAMP/PKA Pathway.” The Journal of Cell Biology 177.6 (2007).

PMCID: 2064370

Applications: Coimmunoprecipitation,

Western Blot

Reactivity: Mouse

Murray, Fiona, et al. "Expression and activity of cAMP phosphodiesterase isoforms in pulmonary artery smooth muscle cells from patients with pulmonary hypertension: role for PDE1." American Journal of Physiology-Lung Cellular and Molecular Physiology 292.1 (2007).

PMID: 16980375

Applications: Western Blot

Reactivity: Human

Gallagher, Helen C., et al. "Valproate activates phosphodiesterase-mediated cAMP degradation: relevance to C6 glioma G1 phase progression." Neurotoxicology and teratology 26.1 (2004).

PMID: 15001216

Applications: Western Blot

Reactivity: Human

Publishing research using PD4C-301AP? Please let us know, we can cite the reference in this datasheet.