PDE8A Antibody

Product no.: PD8A-101AP

$320.00

Description
Publications

General Information
Product	PDE8A Antibody
Description	Affinity Purified Pan Phosphodiesterase 8A Antibody N-epitope
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Bovine, Human, Monkey, Mouse, Rat
Immunogen	Synthetic cyclic peptide from near the N terminus common to all PDE8A variants.
Specificity	This antibody will label only PDE8A variants. This antibody will not cross react with other PDE family members.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.65 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	N-epitope
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:250
Western Blot	1:500-1:2,000
Protein
Overview	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
Molecular Function	3',5'-cyclic-AMP phosphodiesterase activity, Hydrolase, Metal-binding
Subcellular Location	Cytosol
Expression	Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
Structure	Belongs to cyclic nucleotide phosphodiesterase family. PDE8B subfamily. Contains 1 PAC (Pas-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.
Alternative Nomenclature	5''-cyclic phosphodiesterase 8A antibody cAMP specific cyclic nucleotide phosphodiesterase 8A antibody FLJ16150 antibody High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody HsT19550 antibody PDE 8A antibody Phosphodiesterase 8A antibody Weakly similar to 3'5'-cyclic-nucleotide phosphodiesterase antibody

Protein information supplied by UniProt

Fabgennix PDE8A Antibody has been used in 12 Publications
Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015). PMID25578602	Immunohistochemistry; Human
Amanda, G. Vang, et al. "Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac." Biochemical Journal 456.3 (2013): 463-473. PMID24007532	Western Blot; Mouse
Kelly, Michy P., et al. "Select 3′, 5′-cyclic nucleotide phosphodiesterases exhibit altered expression in the aged rodent brain." Cellular signalling 26.2 (2014): 383-397. PMID24184653	Western Blot; Human, Mouse
Kraynik, Stephen M., Robert S. Miyaoka, and Joseph A. Beavo. “PDE3 and PDE4 Isozyme-Selective Inhibitors Are Both Required for Synergistic Activation of Brown Adipose Tissue.” Molecular Pharmacology 83.6 (2013): 1155–1165. PMC3657100	Western Blot; Mouse
Lu, David et al. “Increase in Cellular Cyclic AMP Concentrations Reverses the Profibrogenic Phenotype of Cardiac Myofibroblasts: A Novel Therapeutic Approach for Cardiac Fibrosis.” Molecular Pharmacology 84.6 (2013): 787–793. PMC3834140	Western Blot; Rat
Shimizu-Albergine, Masami et al. “cAMP-Specific Phosphodiesterases 8A and 8B, Essential Regulators of Leydig Cell Steroidogenesis.” Molecular Pharmacology 81.4 (2012): 556–566. PMC3310417	Western Blot; Mouse
Kruse, Lars Schack, Morten Møller, and Christina Kruuse. "Distribution of PDE8A in the nervous system of the Sprague-Dawley Rat." Journal of chemical neuroanatomy 42.3 (2011): 184-191. PMID21821118	Western Blot; Rat
Bol, Sebastiaan M., et al. "Polymorphism in HIV-1 dependency factor PDE8A affects mRNA level and HIV-1 replication in primary macrophages." Virology 420.1 (2011): 32-42. PMID21920574	Western Blot; Human
Patrucco, Enrico et al. “Phosphodiesterase 8A (PDE8A) Regulates Excitation-Contraction Coupling in Ventricular Myocytes.” Journal of molecular and cellular cardiology 49.2 (2010): 330–333. PMC2885478	Immunoprecipitation, Western Blot; Mouse
Sasseville, Maxime et al. “Characterization of Novel Phosphodiesterases in the Bovine Ovarian Follicle.” Biology of Reproduction 81.2 (2009): 415–425. PMC2849824	Western Blot; Bovine
Chen, Chen et al. “PDE8A Genetic Variation, Polycystic Ovary Syndrome and Androgen Levels in Women.” Molecular Human eproduction 15.8 (2009): 459–469. PMC2709315	Confocal Microscopy, Immunofluorescence, Western Blot; Human
Vasta, Valeria, Masami Shimizu-Albergine, and Joseph A. Beavo. “Modulation of Leydig Cell Function by Cyclic Nucleotide Phosphodiesterase 8A.” Proceedings of the National Academy of Sciences of the United States of America 103.52 (2006): 19925–19930. PMC1750886	Immunoprecipitation; Mouse