General Information
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Product
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PERK Antibody
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Description
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Affinity Purified Eukaryotic translation initiation factor 2-alpha kinase 3 Antibody
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Verified Applications
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DB, ELISA, IHC, IP, WB
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Host
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Chicken
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Species Cross Reactivity
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Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 1000-1050 on human Eukaryotic translation initiation factor 2-alpha kinase 3 protein.
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Accession #
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NCBI: NP_004827
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.68-0.70 µg/µl in antibody stabilization buffer
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Immunoglobulin |
IgY |
Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:4,000
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ELISA
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1:4,000
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Immunohistochemistry |
1:50-1:100 |
Immunoprecipitation
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1:150
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Western Blot
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1:500
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Protein
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Uniprot #
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Q9NZJ5
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Overview
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Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
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Molecular Function
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Kinase, Serine/threonine-protein kinase, Transferase
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Subcellular Location
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Endoplasmic reticulum membrane; Single-pass type I membrane protein
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Expression
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Ubiquitous. A high level expression is seen in secretory tissues.
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Structure
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Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3 and MFN2.
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Alternative Nomenclature
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DKFZp781H1925 antibody
E2AK3_HUMAN antibody
EC 2.7.11.1 antibody
Eif2ak3 antibody
Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
Heme regulated EIF2 alpha kinase antibody
HRI antibody
HsPEK antibody
Pancreatic eIF2 alpha kinase antibody
PEK antibody
PRKR like endoplasmic reticulum kinase antibody
WRS antibody
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