Phospho-FGFR2 Antibody BIOTIN

General Information

Product

Phospho-FGFR2 Antibody BIOTIN

Description

BIOTIN-Conjugated Phospho-Fibroblast Growth Factor Receptor 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human

Immunogen

Synthetic peptide taken within amino acid region 520-570 on human Fibroblast Growth Factor receptor 2 protein.

Accession #

NCBI:  XP_011537812.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

BIOTIN-Conjugated

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P21802

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.

Molecular Function

ATP-binding; Fibroblast growth factor binding; Heparin binding; Protein Tyrosine Kinase activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cytoplasmic vesicle

Note: Detected on osteoblast plasma membrane lipid rafts. After ligand binding, the activated receptor is rapidly internalized and degraded.

Isoform 1 : Cell membrane

Expression

Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle.

Structure

Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF6, FGF7, FGF8, FGF9, FGF10, FGF17, FGF18 and FGF22 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Isoform 1 has high affinity for FGF1 and FGF2, but low affinity for FGF7. Isoform 3 has high affinity for FGF1 and FGF7, and has much higher affinity for FGF7 than isoform 1 (in vitro). Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19 and FGF21. Interacts with PLCG1, GRB2 and PAK4.

Alternative Nomenclature

Bacteria expressed kinase antibody

BBDS antibody

BEK antibody

BEK fibroblast growth factor receptor antibody

BFR1 antibody

CD 332 antibody

CEK 3 antibody

CFD1 antibody

Craniofacial dysostosis 1 antibody

Crouzon syndrome antibody

ECT 1 antibody

FGFR 2 antibody

Fibroblast growth factor receptor 2 antibody

Hydroxyaryl protein kinase antibody

Jackson Weiss syndrome antibody

JWS antibody

K SAM antibody

Keratinocyte growth factor receptor 2 antibody

KGFR antibody

KSAM antibody

Pfeiffer syndrome antibody

Protein tyrosine kinase receptor like 14 antibody

soluble FGFR4 variant 4 antibody

TK14 antibody

TK25 antibody

Tyrosylprotein kinase antibody