Phantom Antibody

General Information

Product

Phantom Antibody

Description

Affinity Purified Phantom isoform A Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Phantom protein.

Accession #

NCBI: NP_573319.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VWR5

Overview

Plays a role in the metabolism of insect hormones through ecdysteroid C25-hydroxylase activity.  May also funciton in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxireductase

Subcellular Location

Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane

Expression

First detected at early blastoderm stage 4, and stripes of expression remain through step 10.  Expression is not detectable during germ band retraction and some expression resumes during stae 15-17.  Low levels of expression are detected in larval brain and fat body.

Alternative Nomenclature

Cytochrome P450 306a1 antibody

Dmphm antibody

Exo antibody

EXO1 antibody

Exocephalon antibody

Protein phantom antibody