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General Information
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Product
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Pin1 Antibody
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Description
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Peptidyl-prolyl cis-trans isomerase Pin1 Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Monkey, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 118-168 on human peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein.
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Accession #
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NCBI: XP_011526370.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.56-0.62 µg/µl in antibody stabilization buffer
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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Dot Blot
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1:10,000
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ELISA
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1:10,000
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Immunoprecipitation
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1:200
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Western blot
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1:500
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Protein
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Uniprot #
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UPI0005D038B7
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Overview
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Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920). Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).
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Molecular Function
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GTPase activating protein binding; mitogen-activated protein kinase kinase binding; peptidyl-prolyl cis-trans isomerase activity; phosphoserine binding; phosphothreonine binding
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Subcellular Location
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Nucleus; Nucleus speckle; Cytoplasm
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Expression
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The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.
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Structure
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Interacts with STIL. Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1. Interacts (via WW domain) with ATCAY; upon NGF stimulation. Interacts with PML (isoform PML-4) and BCL-6. Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923).
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Alternative Nomenclature
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DOD antibody
DODO, Drosophila, homolog of antibody
FLJ40239 antibody
FLJ77628 antibody
MGC10717 antibody
NIMA interacting 1 antibody
Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
Pin 1 antibody
PPIase Pin1 antibody
Prolyl isomerase antibody
Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
Protein NIMA interacting 1 antibody
Rotamase Pin1 antibody
UBL5 antibody
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Pin1 Antibody FITC
Pin1 Antibody BIOTIN
Pin1 Blocking Peptide
Pin1 Positive Control
