Phospho-p27 KIP1 Antibody

Product no.: PKIP-180AP
$350.00


General Information

 

 

Product

 

Phospho-p27 KIP1 Antibody

Description

Affinity Purified Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody Extreme C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 148-198 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.

Accession #

GenBank: AAH01971.1


Physical Properties

 

 

Quantity

 

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20C for long term storage


Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunohistochemistry 1:50-1:150

Immunoprecipitation

1:150

Western Blot

1:250


Protein

 

Uniprot #

 

Q9NYG6

Overview

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Molecular Function

Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity

Subcellular Location

Nucleus

Expression

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Structure

Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex.

Alternative Nomenclature

AA408329 antibody

AI843786 antibody

CDKN 1B antibody

CDKN 4 antibody

Cyclin Dependent Kinase Inhibitor 1B antibody

Cyclin dependent kinase inhibitor p27 antibody

KIP 1 antibody

MEN1B antibody

MEN4 antibody

p27 antibody

p27 Kip1 antibody

P27-like cyclin-dependent kinase inhibitor antibody

   

Protein information supplied by UniProt

 

Accessories

Product Note Status Price
Phospho-p27 KIP1 Antibody FITC Phospho-p27 KIP1 Antibody FITC
PKIP.180-FITC $395.00 *
Phospho-p27 KIP1 Antibody BIOTIN Phospho-p27 KIP1 Antibody BIOTIN
PKIP.180-BIOTIN $395.00 *
Phospho-p27 KIP1 Blocking Peptide Phospho-p27 KIP1 Blocking Peptide
P-PKIP.180 $180.00 *
Phospho-p27 KIP1 Positive Control Phospho-p27 KIP1 Positive Control
PC-PKIP $205.00 *
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