Phospho-p27 KIP1 Antibody

Product no.: PKIP-180AP

$375.00

Description

General Information
Product	Phospho-p27 KIP1 Antibody
Description	Affinity Purified Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody Extreme C-epitope
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Chicken, Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 148-198 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.
Accession #	GenBank: AAH01971.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.50-0.75 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	Extreme C-epitope
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:50-1:150
Immunoprecipitation	1:150
Western Blot	1:250
Protein
Uniprot #	Q9NYG6
Overview	Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Molecular Function	Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity
Subcellular Location	Nucleus
Expression	Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Structure	Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex.
Alternative Nomenclature	AA408329 antibody AI843786 antibody CDKN 1B antibody CDKN 4 antibody Cyclin Dependent Kinase Inhibitor 1B antibody Cyclin dependent kinase inhibitor p27 antibody KIP 1 antibody MEN1B antibody MEN4 antibody p27 antibody p27 Kip1 antibody P27-like cyclin-dependent kinase inhibitor antibody

Protein information supplied by UniProt