Phospho-PDE5A Antibody

Product no.: PPD5A-140AP

$375.00

General Information
Product	Phospho-PDE5A Antibody
Description	Affinity Purified Phosphorylated Phosphodiesterase 5A Antibody
Verified Applications	ELISA, ICC, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Bovine, Human, Mouse, Pig, Rat, Sheep
Immunogen	Synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein. Phosphoryalted
Specificity	This antibody will detect only PDE5A protein that has been phosphorylated at the PKG site. Will not detect unphosphorylated PDE5A, or other PDE family members.
Accession #	GenBank: EAX05277.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	1.0-1.25 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:100
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:100
Immunofluorescence	1:100
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	UPI0000EE2B22
Overview	Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
Molecular Function	Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding
Subcellular Location	cytosol
Expression	Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Alternative Nomenclature	5''-cyclic phosphodiesterase antibody CGB PDE antibody cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody cGMP binding/cGMP specific phosphodiesterase antibody cGMP specific phosphodiesterase type 5A antibody CN5A antibody CN5N antibody PDE 5 antibody PDE 5A antibody PDE5A1 antibody Phosphodiesterase 5A antibody Phosphodiesterase 5A cGMP specific antibody Phosphodiesterase isozyme 5 antibody

Protein information supplied by UniProt

Fabgennix Phospho-PDE5A Antibody has been used in 15 Publications
Mous, Daphne S., et al. "Clinically relevant timing of antenatal sildenafil treatment reduces pulmonary vascular remodeling in congenital diaphragmatic hernia." American Journal of Physiology-Lung Cellular and Molecular Physiology 311.4 (2016): L734-L742. PMID: 27521424	IHC; *Rat*
MHerrera, Emilio A., et al. "The role of nitric oxide signaling in pulmonary circulation of highand low-altitude newborn sheep under basal and acute hypoxic conditions." Nitric Oxide, 89, 71-80. PMID: 31063821	Western Blot; *Sheep*
Musicki, Biljana, et al. "Testosterone replacement in transgenic sickle cell mice controls priapic activity and upregulates PDE5 expression and eNOS activity in the penis." Andrology 6.1 (2018): 184-191. PMID29145710	Western Blot; *Mouse*
Nino, Paula Katherina Bautista, et al. "Phosphodiesterase 1 regulation is a key mechanism in vascular aging." Clinical Science (2015): CS20140753. PMID26464516	Western Blot; *Mouse*
Nino, Paula Katherina Bautista, et al. "Phosphodiesterase 1 regulation is a key mechanism in vascular aging." Clinical Science (2015): CS20140753. PMID26464516	Western Blot; *Mouse*
Nino, Paula Katherina Bautista, et al. "Phosphodiesterase 1 regulation is a key mechanism in vascular aging." Clinical Science (2015): CS20140753. PMID26464516	Western Blot; *Mouse*
Rajagopal, Senthilkumar, et al. "Cytokine-Induced S-Nitrosylation of Soluble Guanylyl Cyclase and Expression of Phosphodiesterase 1A Contribute to Dysfunction of Longitudinal Smooth Muscle Relaxation." Journal of Pharmacology and Experimental Therapeutics 352.3 (2015): 509-518.PMID25550199	Western Blot; *Mouse*

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To view the Specification Sheet for Phospho-PDE5A Antibody, please click the link below:

Phospho-PDE5A Antibody | FabGennix

Which phosphorylation site of PDE5A does this Phospho-PDE5A Antibody (PPD5A-140AP) recognize?
1. This antibody detects only PDE5A protein phosphorylated at Serine 148. This Antibody will detect phosphorylation at the PKG site.