Phospho-Src Antibody

General Information

Product

Phospho-Src Antibody

Description

Affinity Purified Phosphorylated Proto-oncogene tyrosine-protein kinase Src Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

N-epitope phosphorylated synthetic peptide taken within amino acid region 200-300 on human Src protein.  Phosphorylated Tyr-215 residue.

Accession #

NCBI:  NP_005408.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P12931

Overview

Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis migration, and transformation

Molecular Function

ATP binding, ephrin receptor binding, heme binding, ion channel binding, enzyme binding, growth factor receptor binding, integrin binding, kinase activity

Subcellular Location

Cell membrane, mitochondrion inner membrane, nucleus, cytoplasm (Note: Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and SH3 domain.)

Expression

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

Alternative Nomenclature

ASV antibody

Avian sarcoma virus antibody

Neuronal CSRC tyrosine specific protein kinase antibody

p60 Src antibody

SRC1 antibody

Tyrosine kinase pp60c src antibody

v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody