Phospho-TICAM2 Antibody

Product no.: PTRAM-140AP

General Information
Product	Phospho-TICAM2 Antibody
Description	Affinity Purified Phosphorylated TRIF-related adapter molecule Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Phosphorylated synthetic peptide corresponding to unique amino acid sequences on TRIF-related adapter molecule protein.
Accession #	GenBank: AAO74498.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.50-0.75 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500-1:750
Protein
Uniprot #	Q86XR7
Overview	Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4.
Molecular Function	phospholipid binding, signal transducer activity
Subcellular Location	Isoform 1 : Cytoplasm. Golgi apparatus. Cell membrane. Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum. Early endosome membrane. Late endosome membrane.
Expression	Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas Isoform 2 is ubiquitously expressed (at lower levels than isoform 1).
Structure	Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs in late endosomes and dirupts the interaction between isoform 1 and TICAM1. Interacts with MYD88; the interaction decreases after IL-18 stimulation in time-dependent manner. Interacts with IL18R1 and IL8RAP.
Alternative Nomenclature	Putative NF-kappa-B-activating protein 502 antibody TIR containing adapter molecule 2 antibody TIR domain containing adaptor protein antibody TIRAP3 antibody TIRP antibody Toll-like receptor adaptor protein 3 antibody Toll/interleukin-1 receptor domain-containing protein antibody TRAM antibody TRIF-related adapter molecule antibody

Protein information supplied by UniProt

FabGennix Phospho-TRIF adapter molecule Antibody has been used in 5 Publications

Majumdar, Tanmay, et al. "Tryptophan-kynurenine pathway attenuates β-catenin-dependent pro-parasitic role of STING-TICAM2-IRF3-IDO1 signalosome in Toxoplasma gondii infection." Cell Death & Disease 10.3 (2019). PMID30770800

Immunoprecipitation, Western Blot; Human

Iampietro, WCtrial et al. "Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection." PLoS pathogens 13.5 (2017). PMID28542576

Western Blot; Human

McGettrick, Anne F. et al. “Trif-Related Adapter Molecule Is Phosphorylated by PKC? During Toll-Like Receptor 4 Signaling.” Proceedings of the National Academy of Sciences of the United States of America 103.24 (2006): 9196–9201. PMC1482589

Western Blot; Human

Fischer, Hans et al. “Pathogen Specific, IRF3-Dependent Signaling and Innate Resistance to Human Kidney Infection.” Ed. Frederick M. Ausubel.PLoS Pathogens 6.9 (2010): e1001109. PMC2944801

Western Blot; Human, Mouse

Keck, Simone, et al. "Absence of TRIF signaling in lipopolysaccharide-stimulated murine mast cells." The Journal of Immunology 186.9 (2011): 5478-5488. PMID21441453

Western Blot; Mouse

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Phospho-TICAM2 Antibody | FabGennix