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General Information
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Product
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RASGRF2 Antibody
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Description
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Affinity Purified Ras-specific guanine nucleotide-releasing factor 2 Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Immunogen
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Synthetic peptide taken within amino acid region 1100-1200 on human RASGRF2 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.58 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DOT Blot
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1:10,000
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ELISA
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1:10,000
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| Immunohistochemistry |
1:50-1:100 |
| Immunoprecipitation |
1:200 |
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Western Blot
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1:500
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Protein
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Uniprot #
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O14827
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Overview
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Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation.
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Molecular Function
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Guanine-nucleotide releasing factor, Rho guayl-nucleotide exchange factor activity
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Subcellular Location
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Cytoplasm, Cell membrane; Peripheral membrane protein, Endoplasmic reticulum membrane; Peripheral membrane protein
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Expression
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Widely expressed with higher expression in the brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung.
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Structure
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Homooligomer and heterooligomer with RASGRF1. Interacts with Ras and RAC1. Interacts in a calcium-dependent manner with calmodulin.
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Alternative Nomenclature
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GRF2 antibody
Ras guanine nucleotide exchange factor 2 antibody
Ras-GRF2 antibody
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RASGRF2 Antibody FITC
RASGRF2 Antibody BIOTIN
RASGRF2 Blocking Peptide
RASGRF2 Positive Control
