RIP140 Antibody

Product no.: RIP140-101AP
$295.00
$265.00


General Information

 

 

Product

 

RIP140 Antibody N-epitope

Description

Receptor interacting protein 140 Antibody

Verified Applications

CM, ELISA, ICC, IHC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on mouse receptor interacting protein 140.

Specificity Recognizes RIP140 at the N terminal region containing the HDAC binding domain.

Accession #

GenBank: AF053062.1


Physical Properties

 

 

Quantity

 

100 µg

Volume

200 µl

Concentration

0.60 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20C for long term storage


Application Protocol

 

Confocal Microscopy

 

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:1,000


Protein

 

Uniprot #

 

Q8CBD1

Overview

Modulates transcriptional repression by nuclear hormone receptors such as NR2C1, thyroid hormone receptor and retinoic acid receptor/RARA. Essential for cumulus expansion and follicle rupture during ovulation. Also controls the balance between fat accumulation and energy expenditure. Positive regulator of the circadian clock gene expression: stimulates transcription of ARNTL/BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC.

Molecular Function

Core promoter sequence-specific DNA binding; Estrogen Receptor binding; Glucocortioid receptor binding; Histone deacetylase binding; Nuclear hormone receptor binding; Receptor binding; Transcription coactivator/corepressor activity

Subcellular Location

Nucleus

Expression

Expressed in the embryonic placenta. In the adult, expression is strong in the testis and brain. Also expressed at a high level in the white adipose tissue. Expressed constantly but at a weaker level in the adult heart, lung, stomach and kidney. Expressed moderately in the skeletal muscle. Expressed at a low level in the adult spleen, liver and brown adipose tissue. Expressed in the ovary at a high level in granulosa cells and at a lower level in the thecal and interstitial compartments.

Structure

Contains at least 4 autonomous repression domains (RD1-4).By similarity

Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors.

The Ligand-dependent nuclear receptor binding region is required for ligand-dependent interaction with RAAR and RXRB homo- and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB.

Alternative Nomenclature

NRIP 1 antibody

Nuclear factor RIP 140 antibody

Nuclear receptor interacting protein 1 antibody

Receptor-interacting protein 140 antibodyRIP140 antibody

   

Protein information supplied by UniProt
 

 

Accessories

Product Note Status Price
RIP140 Antibody FITC RIP140 Antibody FITC
RIP140.101-FITC $350.00 *
RIP140 Antibody BIOTIN RIP140 Antibody BIOTIN
RIP140.101-BIOTIN $350.00 *
RIP140 Blocking Peptide RIP140 Blocking Peptide
P-RIP140.101 $145.00 *
RIP140 Positive Control RIP140 Positive Control
PC-RIP140 $185.00 *
Display accessory details

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