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General Information
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Product
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RIP140 Antibody C-epitope
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Description
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Receptor interacting protein 140 Antibody
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Verified Applications
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CM, ELISA, ICC, IHC, IF, ChIP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Chicken, Human, Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 1050-2000 corresponding to the C-terminal domain of RIP140.
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| Specificity |
Recognizes the C-terminal domain of RIP140 |
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Accession #
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GenBank: AF053062.1
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.60 µg/µl in antibody stabilization buffer
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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Confocal Microscopy
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1:250
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DB
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1:10,000
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ELISA
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1:10,000
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Immunocytochemistry
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1:250
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Immunofluorescence
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1:250
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Immunohistochemistry
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1:250
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Immunoprecipitation
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1:200
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Western Blot
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1:1,000
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Protein
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Uniprot #
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Q8CBD1
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Overview
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Modulates transcriptional repression by nuclear hormone receptors such as NR2C1, thyroid hormone receptor and retinoic acid receptor/RARA. Essential for cumulus expansion and follicle rupture during ovulation. Also controls the balance between fat accumulation and energy expenditure. Positive regulator of the circadian clock gene expression: stimulates transcription of ARNTL/BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC.
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Molecular Function
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Core promoter sequence-specific DNA binding; Estrogen Receptor binding; Glucocortioid receptor binding; Histone deacetylase binding; Nuclear hormone receptor binding; Receptor binding; Transcription coactivator/corepressor activity.
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Subcellular Location
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Nucleus
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Expression
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Expressed in the embryonic placenta. In the adult, expression is strong in the testis and brain. Also expressed at a high level in the white adipose tissue. Expressed constantly but at a weaker level in the adult heart, lung, stomach and kidney. Expressed moderately in the skeletal muscle. Expressed at a low level in the adult spleen, liver and brown adipose tissue. Expressed in the ovary at a high level in granulosa cells and at a lower level in the thecal and interstitial compartments.
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Structure
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Contains at least 4 autonomous repression domains (RD1-4).
Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors.
The Ligand-dependent nuclear receptor binding region is required for ligand-dependent interaction with RAAR and RXRB homo- and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB.
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Alternative Nomenclature
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NRIP 1 antibody
Nuclear factor RIP 140 antibody
Nuclear receptor interacting protein 1 antibody
Receptor-interacting protein 140 antibody
RIP140 antibody
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RIP140 Antibody FITC
RIP140 Antibody BIOTIN
RIP140 Blocking Peptide
RIP140 Positive Control
