RIP140 Antibody FITC

General Information

Product

RIP140 Antibody C-epitope

Description

Receptor interacting protein 140 Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, ChIP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1050-2000 corresponding to the C terminal domain of RIP140. 

Accession #

GenBank: AF053062.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:1,000

Protein

Uniprot #

Q8CBD1

Overview

Modulates transcriptional repression by nuclear hormone receptors such as NR2C1, thyroid hormone receptor and retinoic acid receptor/RARA. Essential for cumulus expansion and follicle rupture during ovulation. Also controls the balance between fat accumulation and energy expenditure. Positive regulator of the circadian clock gene expression: stimulates transcription of ARNTL/BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC.

Molecular Function

Core promoter sequence-specific DNA binding; Estrogen Receptor binding; Glucocortioid receptor binding; Histone deacetylase binding; Nuclear hormone receptor binding; Receptor binding; Transcription coactivator/corepressor activity.

Subcellular Location

Nucleus

Expression

Expressed in the embryonic placenta. In the adult, expression is strong in the testis and brain. Also expressed at a high level in the white adipose tissue. Expressed constantly but at a weaker level in the adult heart, lung, stomach and kidney. Expressed moderately in the skeletal muscle. Expressed at a low level in the adult spleen, liver and brown adipose tissue. Expressed in the ovary at a high level in granulosa cells and at a lower level in the thecal and interstitial compartments.

Structure

Contains at least 4 autonomous repression domains (RD1-4).

Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors.

The Ligand-dependent nuclear receptor binding region is required for ligand-dependent interaction with RAAR and RXRB homo- and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB.

Alternative Nomenclature

NRIP 1 antibody

Nuclear factor RIP 140 antibody

Nuclear receptor interacting protein 1 antibody

Receptor-interacting protein 140 antibody

RIP140 antibody