ROCK1 Antibody

General Information

Product

ROCK1 Antibody

Description

Affinity Purified Rho-associated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region spanning 850-900 on human ROCK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13464

Overview

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Golgi apparatus membrane; Peripheral membrane protein, Cell projection

Expression

Detected in blood platelets.

Structure

Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.

Alternative Nomenclature

coiled-coil-containing protein kinase 1 antibody

NY REN 35 antigen antibody

p160 Rhoassociated coiled coil-forming protein kinase antibody

p160 ROCK1 antibody

PRO0435 antibody

Renal carcinoma antigen NY REN 35 antibody

Rho associated coiled coil containing protein kinase 1 antibody

Rho-alpha kinase antibody

Rho-associated protein kinase 1 antibody

Rok antibody

rokalpha antibody