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General Information
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Product
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ROCK1 Antibody
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Description
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Affinity Purified Rho-associated protein kinase 1 Antibody
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Verified Applications
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ELISA, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human, Monkey, Mouse, Pig, Rat
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Immunogen
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Synthetic peptide taken within amino acid region spanning 850-900 on human ROCK1 protein.
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.57 µg/µl in antibody stabilization buffer
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Form
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Affinity Purified
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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| Immunohistochemistry |
1:50-1:100 |
| Immunoprecipitation |
1:200 |
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Western Blot
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1:500
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Protein
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Uniprot #
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Q13464
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Overview
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Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress.
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Molecular Function
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Kinase, Serine/threonine-protein kinase, Transferase
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Subcellular Location
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Cytoplasm, Golgi apparatus membrane; Peripheral membrane protein, Cell projection
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Expression
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Detected in blood platelets.
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Structure
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Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.
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Alternative Nomenclature
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coiled-coil-containing protein kinase 1 antibody
NY REN 35 antigen antibody
p160 Rhoassociated coiled coil-forming protein kinase antibody
p160 ROCK1 antibody
PRO0435 antibody
Renal carcinoma antigen NY REN 35 antibody
Rho associated coiled coil containing protein kinase 1 antibody
Rho-alpha kinase antibody
Rho-associated protein kinase 1 antibody
Rok antibody
rokalpha antibody
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ROCK1 Antibody FITC
ROCK1 Antibody BIOTIN
ROCK1 Blocking Peptide
ROCK1 Positive Control
