SHD / Shade Antibody

General Information

Product

SHD / Shade Antibody

Description

Affinity Purified Shade, isoform B Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IHC, IF, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster (fruit fly)

Immunogen

Synthetic peptide taken within amino acid region 1-50 on Ecdysone 20-monooxygenase (SHADE) protein.

Accession #

NCBI:  NP_996074.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VUF8

Overview

Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxidoreductase

Subcellular Location

Mitochondrion membrane

Expression

Strong expression by embryonic stage 10 in epidermis, decreases significantly in older embryos. Third instar larvae show expression in the midgut copper cells, Malpighian tubules and fat body. In the adult ovaries, expression is seen in both nurse cells a

Structure

Belongs to the cytochrome P450 family.

Alternative Nomenclature

SH2 domain-containing adapter protein D antibody

SHD antibody

Src homology 2 domain containing transforming protein D antibody Ecdysone 20-monooxygenase