|
General Information
|
|
|
Product
|
TRF2 Antibody Affinity Purified
|
|
Description
|
Affinity Purified Telomeric Repeat-binding Factor 2 Antibody Mid-Region
|
|
Verified Applications
|
ELISA, IMM, WB
|
|
Host
|
Rabbit
|
|
Species Cross Reactivity
|
Human, Monkey, Mouse, Rat
|
|
Immunogen
|
Synthetic peptide taken within amino acid region 200-250 on human Telomeric repeat-binding factor 2 protein.
|
|
Accession #
|
GenBank: AAB81135.1
|
|
Physical Properties
|
|
|
Quantity
|
100 µg
|
|
Volume
|
200 µl
|
|
Concentration
|
0.50 µg/µl in antibody stabilization buffer
|
|
Form
|
Affinity Purified
|
|
Clonality
|
Polyclonal
|
|
Determinant
|
Mid-Region
|
|
Storage
|
-20⁰C for long term storage
|
|
Application Protocol
|
|
DB
|
1:20,000
|
|
ELISA
|
1:20,000
|
|
Immunoprecipitation
|
1:200
|
|
Western Blot
|
1:500-1:750
|
|
Protein
|
|
Uniprot #
|
Q15554
|
|
Overview
|
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
|
|
Molecular Function
|
Chromatin binding; Double-stranded telomeric DNA binding; Protein C-terminus binding; Protein homodimerization activity; Telomeric DNA binding
|
|
Subcellular Location
|
Nucleus; Chromosome > telomere
|
|
Expression
|
Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
|
|
Structure
|
Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct.
|
|
Alternative Nomenclature
|
Telomeric DNA binding protein antibody
Telomeric repeat binding factor 2 antibody
TERF 2 antibody
TRBF 2 antibody
TRF 2 antibody
TTAGGG repeat-binding factor 2 antibody
|
| |
|
TRF2 Antibody FITC
TRF2 Antibody BIOTIN
TRF2 Blocking Peptide
TRF2 Positive Control
