U2AF59 Antibody

Product no.: U2AF-101AP

$285.00

General Information
Product	U2AF59 Antibody
Description	Affinity Purified Splicing factor U2AF 59 kDa subunit Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Saccharomyces cerevisiae (yeast)
Immunogen	Synthetic peptide taken within amino acid region 480-517 on yeast U2AF59 protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.75-1.13 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500-1:750
Protein
Uniprot #	P36629
Overview	Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.
Molecular Function	mRNA binding, snoRNA binding, nucleotide binding
Subcellular Location	Nucleus
Structure	Forms a heterodimer with the U2AF small subunit. Can also form a homodimer. U2AF large subunit (U2AF59), U2AF small subunit (U2AF23) and SF1 (bpb1) interact to form a complex required for complex A formation. Interacts with wat1/pop3.
Alternative Nomenclature	S. pombe splicing factor U2 59 kDa subunit Schzosacharomyces pombe splicing factor U2 auxiliary factor U2 auxiliary factor 59 kDa subunit U2 snRNP auxiliary factor large subunit mis11 prp2

Protein information supplied by UniProt