iNOS Antibody

Product no.: iNOS-112AP

Description

General Information
Product	iNOS Antibody
Description	Affinity Purified Nitric oxide synthase, inducible Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human
Immunogen	Synthetic peptide taken within amino acid region 1064-1114 on human inducible nitric oxide synthase protein.
Specificity	This antibody will detect both iNOS and iNOS2 protein.
Accession #	GenBank: BAA37123.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55-0.75 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P35228
Overview	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Molecular Function	Arginine binding, FMN binding, iron ion binding, NADPH-hemoprotein reductase activity, nitric-oxide synthase activity, receptor binding, heme binding, NADP binding
Subcellular Location	cortical cytoskeleton; cytoplasm; cytosol; intracellular; nucleus; perinuclear region of cytoplasm; peroxisome
Expression	Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Structure	Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.
Alternative Nomenclature	HEP NOS antibody Hepatocyte NOS antibody HEPNOS antibody inducible antibody Inducible nitric oxide synthase antibody Inducible NO synthase antibody Inducible NOS antibody INOS antibody MAC NOS antibody Macrophage NOS antibody Nitric oxide synthase 2 inducible macrophage antibody nitric oxide synthase 2A (inducible, hepatocytes) antibody Nitric oxide synthase antibody Nitric oxide synthase inducible antibody nitric oxide synthase, macrophage antibody NOS 2 antibody NOS antibody NOS2A antibody NOS2A, Inducible, Hepatocyte antibody Peptidyl-cysteine S-nitrosylase NOS2 antibody

Protein information supplied by UniProt